上海研盟生物科技有限公司Anti-Nucleophosmin抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實驗中。說明書隨貨發(fā)送,您也可以直接我司在線客服索取。客服:.
中文名稱:核仁磷酸蛋白抗體
英文名稱:Anti-Nucleophosmin抗體
別 名:B23; B23; MGC104254; NMP1; NMP1; NO38; NPM 1; NPM; NPM_HUMAN; NPM1; Nucleolar Phosphoprotein B23; Nucleolar Phosphoprotein B23; Nucleolar protein NO38; Nucleophosmin (nucleolar phosphoprotein B23 numatrin); Nucleophosmin; Nucleophosmin/B23.2; Nucleophosmin/nucleoplasmin family member 1; Nucleoplasmin Family Member 1; Nucleoplasmin Family Member 1; Numatrin; Numatrin; OTTHUMP00000161024; OTTHUMP00000161025; OTTHUMP00000223397; OTTHUMP00000223398; TRK fused gene.
核仁磷酸蛋白抗體背景介紹:This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
規(guī)格:0.1ml/0.2ml
濃度:1mg/1ml
抗體來源:本公司抗體有兔來源和鼠來源等
克隆類型:兔來源為多抗,鼠來源為單抗
產(chǎn)品類型:一抗
核仁磷酸蛋白抗體,Anti-Nucleophosmin抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
避免反復(fù)凍融,保質(zhì)期一年。
核仁磷酸蛋白抗體,Anti-Nucleophosmin抗體免疫組化問題解答:
1、染色過強?
原 因:抗體濃度過高或孵育時間過長
解決方法:降低抗體滴度、抗體孵育時間:室溫1小時或4度過夜
原 因:孵育溫度過高超過37度
解決方法:一般在室溫20-28度
原 因:DAB顯色時間過長或濃度過高
解決方法:顯色時間不超過5-12分鐘,以顯微鏡下觀察為準(zhǔn)
2、非特異性背景染色?
原 因:操作過程中沖洗不充分
解決方法:每步?jīng)_洗3次每次5分鐘
原 因:組織中含過氧化物酶未阻斷
解決方法:可再配置新鮮3%H2O2封閉孵育時間延長
原 因:組織中含內(nèi)原性*
解決方法:正常非免疫動物血清再封閉
原 因:血清蛋白封閉不充分
解決方法:血清蛋白封閉不充分
相關(guān)優(yōu)質(zhì)產(chǎn)品:
Fibulin 1 抗“衰老關(guān)鍵蛋白”抗體
Fibulin-5 抗“衰老關(guān)鍵蛋白”抗體
Rabbit human Fibrinogen 兔抗可溶性纖維蛋白原 (親和層析純化)
mouse human Fibrinogen 小鼠抗纖維蛋白原 (親和層析純化)
Rabbit human Fibrinogen 兔抗纖維蛋白原
FLG(filaggrin) 絲聚蛋白/中間絲蛋白抗體
FLIP S/L (Flice-like Inhibitory Protein) 凋亡調(diào)節(jié)基因之一抗體
FLIPS (Flice-like Inhibitory Protein S) 凋亡調(diào)節(jié)基因之一抗體(短型)
FN (Fibronectin) 纖維連接蛋白抗體
FOS B (FBJ osteosarcoma oncogene B) FosB抗體
FPR1(formyl peptide receptor 1) 甲酸基肽受體1抗體
FRA1/FOSL1 (Fos related/like antigen 1) FRA1/FOSL1抗體
FRA2/FOSL2(Fos related/like antigen 2) FRA2/FOSL2抗體
fowl typhoid and pullorum disease 雞白痢、雞傷寒抗體
FOXF1(Forkhead box protein F1) 叉頭蛋白F1抗體
FOXJ1/HFH-4(Forkhead Box J1) 插頭蛋白J1抗體
FoxP1(Forkhead box P1) FoxP1(T細(xì)胞轉(zhuǎn)錄因子)抗體
FoxP3 叉頭蛋白3-T細(xì)胞轉(zhuǎn)錄因子
FOXO1/FOXO1A(Forkhead box protein O1) FOXO1A抗體
FOXO3A 轉(zhuǎn)錄因子FOXO3A抗體
Measles virus of fusion protein 抗麻疹病毒抗體(麻疹病毒融合蛋白)
TBX2/T-box2(T-box transcription factor TBX2/T-box protein 2) 血栓素B2抗體(也一種新發(fā)現(xiàn)的抑癌基因)