上海研盟生物科技有限公司Anti-EMP-1抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實(shí)驗(yàn)中。說明書隨貨發(fā)送,您也可以直接我司在線客服索取。客服:.
中文名稱:表皮膜蛋白1抗體
英文名稱:Anti-EMP-1抗體
別 名:CL-20; EMP-1; EMP1; EMP1_HUMAN; Epithelial membrane protein 1; Protein B4B; TMP; Tumor-associated membrane protein.
表皮膜蛋白1抗體背景介紹:Epithelial membrane protein-1 (EMP-1) is a four pass transmembrane protein consisting of 160 amino acids. It is a member of a small family of epithelial membrane proteins. EMP-1 is very similar in structure to its close relative, Peripheral Myelin Protein 22 (PMP22). It is most predominantly expressed in tissues of the gastrointestinal tract but has also been found to be a junctional protein in the liver expressed along the intercellular border. EMP-1 directly interacts with the C-terminus of the P2X7 receptor and may be involved in membrane blebbing. EMP-1 may also be an important regulator in cell communication, signaling, and adhesion. When EMP-1 is overexpressed, cell proliferation is inhibited, S phase is arrested and G1 phase is prolonged in esophogeal cancer cells. EMP-1 may play a role in tumorigenesis and has been identified as a biomarker for gefitinib treatment resistance for patients with lung cancer.
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
規(guī)格:0.1ml/0.2ml
濃度:1mg/1ml
抗體來(lái)源:本公司抗體有兔來(lái)源和鼠來(lái)源等
克隆類型:兔來(lái)源為多抗,鼠來(lái)源為單抗
產(chǎn)品類型:一抗
表皮膜蛋白1抗體,Anti-EMP-1抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
避免反復(fù)凍融,保質(zhì)期一年。
表皮膜蛋白1抗體,Anti-EMP-1抗體免疫組化問題解答:
1、染色過強(qiáng)?
原 因:抗體濃度過高或孵育時(shí)間過長(zhǎng)
解決方法:降低抗體滴度、抗體孵育時(shí)間:室溫1小時(shí)或4度過夜
原 因:孵育溫度過高超過37度
解決方法:一般在室溫20-28度
原 因:DAB顯色時(shí)間過長(zhǎng)或濃度過高
解決方法:顯色時(shí)間不超過5-12分鐘,以顯微鏡下觀察為準(zhǔn)
2、非特異性背景染色?
原 因:操作過程中沖洗不充分
解決方法:每步?jīng)_洗3次每次5分鐘
原 因:組織中含過氧化物酶未阻斷
解決方法:可再配置新鮮3%H2O2封閉孵育時(shí)間延長(zhǎng)
原 因:組織中含內(nèi)原性*
解決方法:正常非免疫動(dòng)物血清再封閉
原 因:血清蛋白封閉不充分
解決方法:血清蛋白封閉不充分
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