上海研盟生物科技有限公司Anti-BAIAP2抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實(shí)驗(yàn)中。說(shuō)明書(shū)隨貨發(fā)送,您也可以直接我司在線(xiàn)客服索取。客服:.
中文名稱(chēng):胰島素受體底物p53蛋白抗體
英文名稱(chēng):Anti-BAIAP2抗體
別 名:Insulin receptor substrate P53; IRSp53; IRS P53; IRS-P53; Baiap2; BAI1 associated protein 2 isoform 3; Brain-specific angiogenesis inhibitor 1-associated protein 2; BAI1-associated protein 2; Insulin receptor tyrosine kinase substrate protein p53; Insulin receptor substrate p53; Insulin receptor substrate protein of 53 kDa; IRSp53; BAIP2_HUMAN; BAI-associated protein 2; BAI1-associated protein 2; Protein BAP2; Fas ligand-associated factor 3; Insulin receptor substrate p53/p58; Insulin receptor substrate protein of 53 kDa; FLAF3; IRS-58; IRSp53/58.
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
胰島素受體底物p53蛋白抗體背景介紹:The protein encoded by this gene has been identified as a brain-specific angiogenesis inhibitor (BAI1)-binding protein. This interaction at the cytoplasmic membrane is crucial to the function of this protein, which may be involved in neuronal growth-cone guidance. This protein functions as an insulin receptor tyrosine kinase substrate and suggests a role for insulin in the central nervous system. This protein has also been identified as interacting with the dentatorubral-pallidoluysian atrophy gene, which is associated with an autosomal dominant neurodegenerative disease. It also associates with a downstream effector of Rho small G proteins, which is associated with the formation of stress fibers and cytokinesis. Alternative splicing of the end of this gene results in three products of undetermined function.
規(guī)格:0.1ml/0.2ml
濃度:1mg/1ml
抗體來(lái)源:本公司抗體有兔來(lái)源和鼠來(lái)源等
克隆類(lèi)型:兔來(lái)源為多抗,鼠來(lái)源為單抗
產(chǎn)品類(lèi)型:一抗
胰島素受體底物p53蛋白抗體,Anti-BAIAP2抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
避免反復(fù)凍融,保質(zhì)期一年。
抗原抗體反應(yīng):
一、抗原抗體反應(yīng)特點(diǎn)
1.高度特異性,特異性是抗原抗體反應(yīng)的zui主要特征,特定抗原決定簇只與特定抗體結(jié)合;
2.可逆性:抗原與抗體結(jié)合形成抗原抗體復(fù)合物的過(guò)程是一種動(dòng)態(tài)平衡, 其反應(yīng)式為:Ag+AbAg·Ab;
3.適當(dāng)濃度和比例;
4.敏感性:在測(cè)定血清中某一物質(zhì)的含量時(shí),化學(xué)比色法的敏感度為mg/ml水平,酶反應(yīng)測(cè)定法的敏感度約為5~10μg/ml,免疫測(cè)定中凝膠擴(kuò)散法的敏感度與酶反應(yīng)法相仿。
二、影響抗原抗體反應(yīng)的因素
1.電解質(zhì) :抗原抗體特異結(jié)合后,膠體粒便可發(fā)生脫水作用,此時(shí)易受電解質(zhì)影響,如有適當(dāng)濃度的電解質(zhì)存在就會(huì)使它們失去部分負(fù)電荷而相互凝聚,于是出現(xiàn)明顯的凝集或沉淀現(xiàn)象。
2.酸堿度:合適的pH為6-8。
3.溫度:37℃通常是抗原體反應(yīng)zui適溫度。
胰島素受體底物p53蛋白抗體,Anti-BAIAP2抗體免疫組化問(wèn)題解答:
1、染色過(guò)強(qiáng)?
原 因:抗體濃度過(guò)高或孵育時(shí)間過(guò)長(zhǎng)
解決方法:降低抗體滴度、抗體孵育時(shí)間:室溫1小時(shí)或4度過(guò)夜
原 因:孵育溫度過(guò)高超過(guò)37度
解決方法:一般在室溫20-28度
原 因:DAB顯色時(shí)間過(guò)長(zhǎng)或濃度過(guò)高
解決方法:顯色時(shí)間不超過(guò)5-12分鐘,以顯微鏡下觀察為準(zhǔn)
2、非特異性背景染色?
原 因:操作過(guò)程中沖洗不充分
解決方法:每步?jīng)_洗3次每次5分鐘
原 因:組織中含過(guò)氧化物酶未阻斷
解決方法:可再配置新鮮3%H2O2封閉孵育時(shí)間延長(zhǎng)
原 因:組織中含內(nèi)原性*
解決方法:正常非免疫動(dòng)物血清再封閉
原 因:血清蛋白封閉不充分
解決方法:血清蛋白封閉不充分
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