中文名稱:EB病毒LMP-2A蛋白抗體
英文名稱:Anti-LMP2A antibody
EB病毒LMP-2A蛋白抗體,Anti-LMP2A抗體背景介紹:Epstein Barr virus (EBV) is a member of the herpesvirus family and one of the most common human viruses. Most people become infected with EBV during their lives. Primary infections usually results in infectious mononucleosis (glandular fever) but the virus can also lay dormant in B lymphocytes and when reactivated become associated with more serious disease such as Burkitt's lymphoma, nasopharyngeal carcinoma and Hodgkin's disease. EBV latently infects B lymphocytes. Infected B cells express EBV nuclear antigens and latent proteins LMP1, LMP2A and LMP2B. LMP2A forms aggregates in the plasma membranes of B lymphocytes, where it functions as a negative regulator of the Src and Syk protein tyrosine kinases. Studies show that LMP2A blocks B-cell receptor (BCR) signal transduction in EBV immortalized B cells in vitro and may play an important role in maintaining a latent EBV infection within the peripheral blood B cells of infected individuals.
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相關(guān)標(biāo)記抗體:HRP標(biāo)記抗體,Biotin標(biāo)記抗體,Gold標(biāo)記抗體,RBITC標(biāo)記抗體,AP標(biāo)記抗體,FITC標(biāo)記抗體,Cy3標(biāo)記抗體,Cy5標(biāo)記抗體,Cy5.5標(biāo)記抗體,Cy7標(biāo)記抗體,PE標(biāo)記抗體,PE-Cy3標(biāo)記抗體,PE-Cy5標(biāo)記抗體,PE-Cy5.5標(biāo)記抗體,PE-Cy7標(biāo)記抗體,APC標(biāo)記抗體,Alexa Fluor 350標(biāo)記抗體,Alexa Fluor 488標(biāo)記抗體,Alexa Fluor 555標(biāo)記抗體,Alexa Fluor 647標(biāo)記抗體
保質(zhì)期: 1年
EB病毒LMP-2A蛋白抗體,Anti-LMP2A抗體簡單介紹:
產(chǎn)品別名:EBV latent membrane protein 2A; Epstein Barr virus; HHV4; Human Herpesvirus 4; Latent membrane protein 2; LMP2; Terminal protein; LMP2A; LMP2_EBVB9.
抗體來源:Rabbit or Mouse
克隆類型:Polyclonal or monoclonal
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
EB病毒LMP-2A蛋白抗體抗原抗體反應(yīng):
一、抗原抗體反應(yīng)特點(diǎn)
1.高度特異性,特異性是抗原抗體反應(yīng)的zui主要特征,特定抗原決定簇只與特定抗體結(jié)合;
2.可逆性:抗原與抗體結(jié)合形成抗原抗體復(fù)合物的過程是一種動(dòng)態(tài)平衡, 其反應(yīng)式為:Ag+AbAg·Ab;
3.適當(dāng)濃度和比例;
4.敏感性:在測定血清中某一物質(zhì)的含量時(shí),化學(xué)比色法的敏感度為mg/ml水平,酶反應(yīng)測定法的敏感度約為5~10μg/ml,免疫測定中凝膠擴(kuò)散法的敏感度與酶反應(yīng)法相仿。
二、影響抗原抗體反應(yīng)的因素
1.電解質(zhì) :抗原抗體特異結(jié)合后,膠體粒便可發(fā)生脫水作用,此時(shí)易受電解質(zhì)影響,如有適當(dāng)濃度的電解質(zhì)存在就會(huì)使它們失去部分負(fù)電荷而相互凝聚,于是出現(xiàn)明顯的凝集或沉淀現(xiàn)象。
2.酸堿度:合適的pH為6-8。
3.溫度:37℃通常是抗原體反應(yīng)zui適溫度。
免疫組化染色失敗的幾種原因
1.所染的全部切片均為陰性結(jié)果,包括陽性對(duì)照在內(nèi),全部呈陰性反應(yīng),原因可能是:(1)染色未按嚴(yán)格操作步驟進(jìn)行;(2)漏加一種抗體,或抗體失效;(3)緩沖液內(nèi)含*,抑制了酶的活性;(4)底物中所加H2O2量少或失效;復(fù)染或脫水劑使用不當(dāng)。
2.所有切片均呈弱陽性反應(yīng):(1)切片在染色過程中抗體過濃,或干燥了;(2)緩沖液配制中未加氯化鈉或pH不準(zhǔn)確,洗滌不*;(3)使用已變色的呈色底物溶液,或呈色反應(yīng)時(shí)間過長;(4)抗體溫育的時(shí)間過長;(5)H2O2濃度過高,呈色速度過快;(6)粘附劑太厚。
3.所有切片背景過深:(1)未用酶消化處理切片;(2)切片或涂片過厚;(3)漂洗不夠;(4)底物呈色反應(yīng)過久;(5)蛋白質(zhì)封閉不夠或所用血清溶血;(6)使用全血清抗體稀釋不夠。
4.陽性對(duì)照染色良好,檢測的陽性標(biāo)本呈陰性反應(yīng),固定和處理不當(dāng)是zui常見的原因。對(duì)于陽性結(jié)果的定量判斷,常規(guī)方法是根據(jù)呈色深淺和陽性細(xì)胞數(shù)量分類計(jì)數(shù),以(-)、+、++、+++等分級(jí)和計(jì)數(shù)統(tǒng)計(jì)。
新學(xué)期開學(xué)大減價(jià)*:
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