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上海研盟生物科技有限公司Anti-CLASP2抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實(shí)驗(yàn)中。說(shuō)明書隨貨發(fā)送,您也可以直接我司在線客服索取??头?/span>
英文名稱:Anti-CLASP2 antibody
中文名稱:細(xì)胞連接相關(guān)蛋白2抗體
細(xì)胞連接相關(guān)蛋白2抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
★研盟生物★細(xì)胞連接相關(guān)蛋白2抗體,Anti-CLASP2抗體
抗原修復(fù),必須注意以下問(wèn)題:
1、修復(fù)液的選擇。
0.01M枸櫞酸(pH6.0):應(yīng)用zui多的抗原修復(fù)液,適用于大多數(shù)的抗原,用該液修復(fù)后的抗原表達(dá)增強(qiáng),抗原的定性和定位很理想,結(jié)果不錯(cuò);
0.05M EDTA(pH8.0):應(yīng)用較多的抗原修復(fù)液,修復(fù)能力較強(qiáng),對(duì)于保存時(shí)間較長(zhǎng)的切片或目的蛋白表達(dá)較弱的組織有很好的修復(fù)效果,需要控制好修復(fù)時(shí)間,否則容易出現(xiàn)較重的背景;
0.01M TBS(pH7.4):中性修復(fù)液,適用于大多數(shù)抗原,對(duì)于核定位蛋白有較好的修復(fù)效果;
*:應(yīng)用較多的抗原修復(fù)液,適用于大多數(shù)的抗原,使用溫度37℃,對(duì)于容易脫片的切片有很好的保護(hù)作用。
*:應(yīng)用較多的抗原修復(fù)液,適用于大多數(shù)的抗原
2.抗原熱修復(fù)時(shí)應(yīng)選擇的溫度。
據(jù)實(shí)驗(yàn)認(rèn)為溫度在92℃-98℃是合適的,尤其在95℃為,這是因?yàn)椋海?)這種溫度未達(dá)沸騰,切片不容易脫離載玻片;(2)能夠解離和破壞與蛋白交聯(lián)的甲醛醛鍵等,處理時(shí)可以隨意選擇和確定抗原修復(fù)的作用時(shí)間。如果選擇高壓修復(fù),因?yàn)闇囟容^高,時(shí)間不宜過(guò)長(zhǎng)
3.抗原修復(fù)時(shí)有效溫度所需持續(xù)時(shí)間根據(jù)各單位的設(shè)備條件以及使用的儀器不同,抗原修復(fù)時(shí)在有效的溫度范圍內(nèi)所持續(xù)的時(shí)間也不一樣。
4.抗原修復(fù)液必須遵循自然降溫規(guī)律,否則效果不好或達(dá)不到抗原修復(fù)的目的。
5.盡量使用足量的抗原修復(fù)液,防止切片干涸。
6.切片必須附貼牢固,否則發(fā)生掉片。
細(xì)胞連接相關(guān)蛋白2抗體,Anti-CLASP2抗體溶解方法:
方法1:我們的抗體(凍干粉)已經(jīng)包含了0.01M PBS、1% BSA和0.1% 防腐劑(疊氮鈉或*),您只需要加入滅菌的雙蒸水就行了。抗體溶解后,置于-20℃保存,分裝后使用,避免反復(fù)凍融,可保證至少1年有效;
方法2:也可以只加入一半體積的雙蒸水,再用一半體積的甘油補(bǔ)足,置于-20℃保存,這樣抗體不會(huì)凍,有利于抗體的穩(wěn)定。
后續(xù)試驗(yàn)時(shí),再使用對(duì)應(yīng)的緩沖液稀釋成工作液,即用即配。
我們的抗體(凍干粉),在常溫放置下,至少一個(gè)月有效;若在-20℃下保存(*),至少三年有效。
Antibodies are prepared with 0.01M PBS, pH 7.4 with 1% BSA and 0.1% Sodium Azide, prior to lyophilization. Currently, we offer both lyophilized and reconstituted antibodies, both resulting in final concentration of 1mg/mL. We recommend two ways to reconstitute antibodies in lyophilized form:
1) Reconstituting by directly adding 100uL of sterile, distilled water.
2) Alternatively, if glycerol does not affect the experiment, add 50uL sterile distilled water, followed by adding 50uL of glycerol.
簡(jiǎn)單介紹:
產(chǎn)品別名:CLASP 2; CLIP associating protein 2; Cytoplasmic linker associated protein 2; hOrbit2; KIAA0627; Multiple asters (Mast) like homolog 2; Protein Orbit homolog 2; CLAP2_HUMAN.
抗體來(lái)源:Rabbit or Mouse
保質(zhì)期:1年
克隆類型:Polyclonal or monoclonal
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
背景介紹:CLASP2 is a microtubule plus end tracking protein that promotes the stabilization of dynamic microtubules and is required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. CLASP2 may act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3 kinase signaling. CLASP2 also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle.
相關(guān)優(yōu)質(zhì)產(chǎn)品:
IRF-4(Interferon regulatory factor 4) 干擾素調(diào)節(jié)因子4抗體
ILT2/CD85j(Immunoglobulin-like transcript 2) 細(xì)胞表面免疫球蛋白樣轉(zhuǎn)錄因子2抗體
IBYK P53 (Insulin receptor substrate P53) 胰島素受體底物p53蛋白抗體
IBYK-1 胰島素受體底物-1抗體
IBYK-2 胰島素受體底物-2抗體
IBYK-3 胰島素受體底物-3抗體
IBYK-4 胰島素受體底物-4抗體
ISR(Insulin Receptor)(hu, cattle, mo, rat, hoBYKe, sheep, Rb, dog, chi) 胰島素受體抗體
ISR-α (Insulin Receptor-α) 胰島素受體-α抗體
ISR-β (Insulin Receptor-β) 胰島素受體-β抗體
Integrin α4/CD49d(Integrin alpha 4) 整合素α4抗體
Integrin a7(integrin alpha 7) 整合素α7抗體
Integrin β1/CD29(integrin beta 1) 整合素β1抗體
Integrin α5/CD49e/VLA-5(Integrin αV) 整合素α5抗體
Integrin α6/CD49f/VLA-6(Integrin alpha6/Laminin Receptor) 整合素α6抗體
Integrin β3/CD61(Integrin beta 3) 整合素β3抗體
JAK1(Tyrosine-protein kinase JAK1; Janus kinase 1) 蛋白質(zhì)*激酶JAK-1抗體
JAK2(Tyrosine-protein kinase JAK2; Janus kinase 2; JAK-2) 蛋白質(zhì)*激酶JAK-2抗體