上海研盟生物有限公司*品質(zhì)供應(yīng)Anti-PQBP1抗體,我公司銷售國(guó)內(nèi)外科研產(chǎn)品,如生物試劑、生化試劑,一抗二抗,各種標(biāo)記抗體,Elisa酶聯(lián)免疫試劑盒,放免試劑盒,培養(yǎng)基,各種動(dòng)物血清等產(chǎn)品,歡迎廣大新老客戶前來咨詢訂購,訂購:或。
中文名稱:多*結(jié)合蛋白1抗體
英文名稱:Anti-PQBP1抗體
避免反復(fù)凍融,保質(zhì)期一年。
多*結(jié)合蛋白1抗體,Anti-PQBP1抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
各種HRP酶標(biāo)記成抗體的效價(jià)測(cè)定程序如下(如:羊抗鼠IgG-HRP的效價(jià)測(cè)定):
1、抗原包板:15ug/ml 小鼠IgG-pH9.6·CB液 0.1ml/孔;
2、37°C包被60min或4°C過夜;
3、PBST洗板3次,5min/次;
4、加入倍比稀釋的HRP標(biāo)記抗體 0.1ml/孔;
5、37°C 30min;
6、PBST洗板5min/次×3;
7、底物液0.1ml/孔 37°C 5-10min;
8、終止液:2N H2SO4 0.05ml/孔;
9、450nm 測(cè)定OD值;
10、陽性O(shè)D值比對(duì)照值大3倍,為判斷效價(jià)稀釋度。
多*結(jié)合蛋白1抗體,Anti-PQBP1抗體簡(jiǎn)單介紹
別名:38 kDa nuclear protein containing a WW domain; Mental retardation, X linked 55; MRX55; MRXS3; MRXS8; Npw38; Nuclear protein containing WW domain 38 kD; Polyglutamine binding protein 1; Polyglutamine tract binding protein 1; Polyglutamine tract-binding protein 1; Polyglutamine-binding protein 1; PQBP 1; PQBP-1; PQBP1; PQBP1_HUMAN; RENS1; SHS; Sutherland Haan X linked mental retardation syndrome.
規(guī)格:0.1ml/0.2ml
濃度:1mg/1ml
抗體來源:本公司抗體有兔來源和鼠來源等
克隆類型:兔來源為多抗,鼠來源為單抗
產(chǎn)品類型:一抗
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
多*結(jié)合蛋白1抗體背景介紹:Polyglutamine(Q) tract binding protein-1 (PQBP-1) is a transcription repressor that associates with polyglutamine tract-containing transcription regulators and causative genes for neurodegenerative disorders. Hepta- and di-amino acid repeat sequences rich in polar residues are essential for PQBP-1 to interact with polyglutamine tract-containing proteins (i.e. huntingtin, androgen receptor and Brain-2). PQBP-1 contains a WWP/WW domain that binds proline-rich motifs and a C2 domain that can influence Ca2+-dependent phospholipid signaling. PQBP-1 localizes to the nucleus and is present in neurons throughout the brain, with abundant levels in hippocampus, cerebellar cortex and olfactory bulb. The human PQBP-1 gene maps to chromosome Xp11.23.
免疫熒光染色與免疫組化前期處理相同,因?yàn)閮?nèi)源性過氧化物酶對(duì)免疫熒光實(shí)驗(yàn)沒有影響,同時(shí)雙氧水反而會(huì)干擾到熒光素發(fā)光,所以這步操作在免疫熒光中不需要做。
常用的熒光顯微鏡一般有三個(gè)通路,激發(fā)波長(zhǎng)為350nm、488nm、555nm附近,分別對(duì)應(yīng)的熒光發(fā)射光顏色為藍(lán)光、綠光、紅光。
350nm常用熒光素:DAPI(復(fù)染用),Alexa Fluor 350
488nm常用熒光素:FITC,Alexa Fluor 488
555nm常用熒光素:PE、Cy3、Rhodamine B、Alexa Fluor555
另有其他常見熒光素
594nm常用熒光素:Texas Red、Alexa Fluor594
647nm常用熒光素:Cy5、APC、Alexa Fluor647
相關(guān)優(yōu)質(zhì)產(chǎn)品:
Mfn1 (Mitofusin1) 線粒體融合蛋白1抗體
MGMT (O6-metYMlguanine-DNA metYMltransferase) O6甲基*DNA甲基轉(zhuǎn)移酶抗體
MT(metallothionein) 金屬硫蛋白抗體
MGr1-Ag/37LRP(P37-kDa laminin receptor precuBYKor)(NT) 層粘連蛋白受體1抗體(N端)
MTA1(Metastasis-associated 1) 腫瘤轉(zhuǎn)移相關(guān)蛋白1抗體
MGr1-Ag/37LRP(P37-kDa laminin receptor precuBYKor)(CT) 層粘連蛋白受體1抗體(C端)
Myf6(Myogenic factor 6) 生肌調(diào)節(jié)因子6抗體
MGr1-Ag monoclonal antibody(laminin receptor 1; 67kD, ribosomal protein SA) 小鼠抗層粘連蛋白受體1單克隆抗體
MICA(MHC class I polypeptide-related sequence A) MHC I類鏈相關(guān)蛋白A抗體
Microsporidia 蜜蜂微孢子蟲蛋白抗體
Midnolin isoform Protein 1 中腦核仁蛋白1抗體
Midnolin isoform Protein 2 中腦核仁蛋白2抗體
MK(Midkine) 中期因子/肝素結(jié)合生長(zhǎng)因子抗體
MLCK(myosin light chain kinase) 肌球蛋白輕鏈激酶抗體
MIF (Macrophage Migration Inhibitory Factor) 巨噬細(xì)胞移動(dòng)抑制因子抗體
MIP-1α (macrophage inflammatory protein 1α) 巨噬細(xì)胞炎癥因子1α抗體
MIP-1β (macrophage inflammatory protein 1β) 巨噬細(xì)胞炎癥因子1β 抗體
MIP4/CCL18(Macrophage inflammatory protein 4) 巨噬細(xì)胞炎癥蛋白18抗體