中文名稱:賴氨酸羥化酶2抗體
英文名稱:Anti-PLOD2 antibody
賴氨酸羥化酶2抗體背景介紹:The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. Mutations in the coding region of this gene are associated with Bruck syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.
上海研盟生物科技有限公司Anti-PLOD2抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實(shí)驗(yàn)中。說明書隨貨發(fā)送,您也可以直接我司在線客服索取??头?.(添加索取產(chǎn)品說明與報(bào)價(jià))
相關(guān)標(biāo)記抗體:HRP標(biāo)記抗體,Biotin標(biāo)記抗體,Gold標(biāo)記抗體,RBITC標(biāo)記抗體,AP標(biāo)記抗體,FITC標(biāo)記抗體,Cy3標(biāo)記抗體,Cy5標(biāo)記抗體,Cy5.5標(biāo)記抗體,Cy7標(biāo)記抗體,PE標(biāo)記抗體,PE-Cy3標(biāo)記抗體,PE-Cy5標(biāo)記抗體,PE-Cy5.5標(biāo)記抗體,PE-Cy7標(biāo)記抗體,APC標(biāo)記抗體,Alexa Fluor 350標(biāo)記抗體,Alexa Fluor 488標(biāo)記抗體,Alexa Fluor 555標(biāo)記抗體,Alexa Fluor 647標(biāo)記抗體
保質(zhì)期: 1年
賴氨酸羥化酶2抗體,Anti-PLOD2抗體簡(jiǎn)單介紹:
產(chǎn)品別名:2-oxoglutarate 5-dioxygenase 2; LH2; Lysine hydroxylase 2; Lysyl hydroxylase 2; OTTHUMP00000215204; OTTHUMP00000215205; OTTHUMP00000215206; PLOD 2; Plod2; PLOD2_HUMAN; Procollagen lysine 2 oxoglutarate 5 dioxygenase 2; Procollagen lysine, 2 oxoglutarate 5 dioxygenase (lysine hydroxylase) 2; Procollagen-lysine; opeptide lysyl hydroxylase; TLH.
抗體來源:Rabbit or Mouse
克隆類型:Polyclonal or monoclonal
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
賴氨酸羥化酶2抗體,Anti-PLOD2抗體抗原抗體反應(yīng)
一、抗原抗體反應(yīng)特點(diǎn)
1.高度特異性,特異性是抗原抗體反應(yīng)的zui主要特征,特定抗原決定簇只與特定抗體結(jié)合;
2.可逆性:抗原與抗體結(jié)合形成抗原抗體復(fù)合物的過程是一種動(dòng)態(tài)平衡, 其反應(yīng)式為:Ag+AbAg·Ab;
3.適當(dāng)濃度和比例;
4.敏感性:在測(cè)定血清中某一物質(zhì)的含量時(shí),化學(xué)比色法的敏感度為mg/ml水平,酶反應(yīng)測(cè)定法的敏感度約為5~10μg/ml,免疫測(cè)定中凝膠擴(kuò)散法的敏感度與酶反應(yīng)法相仿。
二、影響抗原抗體反應(yīng)的因素
1.電解質(zhì) :抗原抗體特異結(jié)合后,膠體粒便可發(fā)生脫水作用,此時(shí)易受電解質(zhì)影響,如有適當(dāng)濃度的電解質(zhì)存在就會(huì)使它們失去部分負(fù)電荷而相互凝聚,于是出現(xiàn)明顯的凝集或沉淀現(xiàn)象。
2.酸堿度:合適的pH為6-8。
3.溫度:37℃通常是抗原體反應(yīng)zui適溫度。
免疫組化染色失敗的幾種原因
1.所染的全部切片均為陰性結(jié)果,包括陽性對(duì)照在內(nèi),全部呈陰性反應(yīng),原因可能是:(1)染色未按嚴(yán)格操作步驟進(jìn)行;(2)漏加一種抗體,或抗體失效;(3)緩沖液內(nèi)含*,抑制了酶的活性;(4)底物中所加H2O2量少或失效;復(fù)染或脫水劑使用不當(dāng)。
2.所有切片均呈弱陽性反應(yīng):(1)切片在染色過程中抗體過濃,或干燥了;(2)緩沖液配制中未加氯化鈉或pH不準(zhǔn)確,洗滌不*;(3)使用已變色的呈色底物溶液,或呈色反應(yīng)時(shí)間過長;(4)抗體溫育的時(shí)間過長;(5)H2O2濃度過高,呈色速度過快;(6)粘附劑太厚。
3.所有切片背景過深:(1)未用酶消化處理切片;(2)切片或涂片過厚;(3)漂洗不夠;(4)底物呈色反應(yīng)過久;(5)蛋白質(zhì)封閉不夠或所用血清溶血;(6)使用全血清抗體稀釋不夠。
4.陽性對(duì)照染色良好,檢測(cè)的陽性標(biāo)本呈陰性反應(yīng),固定和處理不當(dāng)是zui常見的原因。對(duì)于陽性結(jié)果的定量判斷,常規(guī)方法是根據(jù)呈色深淺和陽性細(xì)胞數(shù)量分類計(jì)數(shù),以(-)、+、++、+++等分級(jí)和計(jì)數(shù)統(tǒng)計(jì)。
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