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上海研盟生物科技有限公司Anti-ERO1L抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實(shí)驗(yàn)中。說明書隨貨發(fā)送,您也可以直接我司在線客服索取??头?/span>
英文名稱:Anti-ERO1L antibody
中文名稱:內(nèi)質(zhì)網(wǎng)氧化物蛋白Ero1-Lα抗體
內(nèi)質(zhì)網(wǎng)氧化物蛋白Ero1-Lα抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
★研盟生物★內(nèi)質(zhì)網(wǎng)氧化物蛋白Ero1-Lα抗體,Anti-ERO1L抗體
免疫組化操作步驟
(一)、儀器設(shè)備
1、 18cm不銹鋼高壓鍋或電爐或用微波爐
2、 水浴鍋
(二)、試 劑
1、 PBS緩沖液(pH7.2―7.4)
2、 0.01mol/L檸檬酸鈉緩沖液(CB,pH6.0,1000ml)
3、 3%甲醇―H2O2溶液:用30% H2O2 和80%甲醇溶液配制
4、 風(fēng)裱劑:
a. 甘油和0.5mmol/L碳酸鹽緩沖液(pH9.0–9.5)等量混合
b. 油和TBS(PBS)配制
5、 TBS/PBS pH9.0–9.5,適用于熒光纖維鏡標(biāo)本;pH7.0-7.4適合光學(xué)纖維標(biāo)本
(三)、操作流程
1、 脫蠟和水化:脫蠟前應(yīng)將組織芯片在室溫中放置60分鐘或60℃恒溫箱中烘烤20分鐘
a. 組織芯片置于二甲苯中浸泡10分鐘,更換二甲苯后在浸泡10分鐘
b. 無水乙醇中浸泡五分鐘
c. 95%乙醇中浸泡五分鐘
d. 75%乙醇中浸泡五分鐘
2、 抗原修復(fù):用于福爾馬林固定的石蠟包埋組織芯片
內(nèi)質(zhì)網(wǎng)氧化物蛋白Ero1-Lα抗體,Anti-ERO1L抗體溶解方法:
方法1:我們的抗體(凍干粉)已經(jīng)包含了0.01M PBS、1% BSA和0.1% 防腐劑(疊氮鈉或*),您只需要加入滅菌的雙蒸水就行了??贵w溶解后,置于-20℃保存,分裝后使用,避免反復(fù)凍融,可保證至少1年有效;
方法2:也可以只加入一半體積的雙蒸水,再用一半體積的甘油補(bǔ)足,置于-20℃保存,這樣抗體不會(huì)凍,有利于抗體的穩(wěn)定。
后續(xù)試驗(yàn)時(shí),再使用對(duì)應(yīng)的緩沖液稀釋成工作液,即用即配。
我們的抗體(凍干粉),在常溫放置下,至少一個(gè)月有效;若在-20℃下保存(*),至少三年有效。
Antibodies are prepared with 0.01M PBS, pH 7.4 with 1% BSA and 0.1% Sodium Azide, prior to lyophilization. Currently, we offer both lyophilized and reconstituted antibodies, both resulting in final concentration of 1mg/mL. We recommend two ways to reconstitute antibodies in lyophilized form:
1) Reconstituting by directly adding 100uL of sterile, distilled water.
2) Alternatively, if glycerol does not affect the experiment, add 50uL sterile distilled water, followed by adding 50uL of glycerol.
內(nèi)質(zhì)網(wǎng)氧化物蛋白Ero1-Lα抗體,Anti-ERO1L抗體簡單介紹:
產(chǎn)品別名:Endoplasmic oxidoreductin 1 like protein; Endoplasmic oxidoreductin-1-like protein; ERO1 alpha; ERO1 L; ERO1 Lalpha; ERO1 like protein alpha; ERO1-L; ERO1-L-alpha; ERO1-like (S. cerevisiae); ERO1-like protein alpha; ERO1A; ERO1A_HUMAN; ERO1L; Oxidoreductin 1 Lalpha; Oxidoreductin-1-L-alpha.
抗體來源:Rabbit or Mouse
保質(zhì)期:1年
克隆類型:Polyclonal or monoclonal
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
背景介紹:Ero1-La is an essential oxidoreductase that oxidizes proteins and is required for the folding of immunoglobulins. Ero-1La covalently binds with PDI (protein disulfide-isomerase) and together they produce disulfide bonds between proteins in the endoplasmic reticulum. Ero1-La and SIRT1 regulate adiponectin secretion from adipose tissue. Ero1-La and associated proteins also modulate PPARg (peroxisome proliferator-activated receptor g) and SIRT1 activities. Ero1-La is stimulated by hypoxia, suggesting that it is regulated through the HIF (hypoxia inducible transcrip-tion factor) pathway. Ero1-La is ubiquitously expressed at low levels but expressed at high levels in upper digestive tract and esophagus. Ero1-La may function both as a monomer and a homodimer.
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