上海研盟生物有限公司*品質(zhì)供應(yīng)Anti-LAP3抗體，我公司銷(xiāo)售國(guó)內(nèi)外科研產(chǎn)品，如生物試劑、生化試劑，一抗二抗，各種標(biāo)記抗體，Elisa酶聯(lián)免疫試劑盒，放免試劑盒，培養(yǎng)基，各種動(dòng)物血清等產(chǎn)品，歡迎廣大新老客戶(hù)前來(lái)咨詢(xún)訂購(gòu)，訂購(gòu):或。

*氨基肽酶3抗體,Anti-LAP3抗體簡(jiǎn)單介紹：

中文名稱(chēng)：*氨基肽酶3抗體

英文名稱(chēng)：Anti-LAP3抗體

別 名：Cytosol aminopeptidase; LAP 3; LAP; LAPEP; Leucine aminopeptidase 3; Leucyl aminopeptidase; PEPS; Peptidase S; Proline aminopeptidase; Prolyl aminopeptidase; AMPL_HUMAN.    

此產(chǎn)品本公司有標(biāo)記的一抗出售，相關(guān)標(biāo)記有：Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記

背景介紹：LAP3 (leucine aminopeptidase 3), also known as LAPEP or PEPS, is a 519 amino acid protein that localizes to the cytoplasm and belongs to the peptidase M17 family. Existing as a homohexamer, LAP3 uses zinc as a cofactor to catalyze the release of an N-terminal proline from a target peptide and is, therefore, involved in the processing and turnover of intracellular proteins. Multiple isoforms of LAP3 exist due to alternative splicing events. The gene encoding LAP3 maps to human chromosome 4, which houses nearly 6% of the human genome and has the largest gene deserts (regions of the genome with no protein encoding genes) of all of the human chromosomes. Defects in some of the genes located on chromosome 4 are associated with Huntington's disease, Ellis-van Creveld syndrome, methylmalonic acidemia and polycystic kidney disease.

規(guī)格：0.1ml/0.2ml

濃度：1mg/1ml

抗體來(lái)源：本公司抗體有兔來(lái)源和鼠來(lái)源等

克隆類(lèi)型：兔來(lái)源為多抗，鼠來(lái)源為單抗

產(chǎn)品類(lèi)型：一抗

*氨基肽酶3抗體保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

避免反復(fù)凍融，保質(zhì)期一年。

*氨基肽酶3抗體,Anti-LAP3抗體免疫組化問(wèn)題解答

1、染色過(guò)強(qiáng)？

原 因：抗體濃度過(guò)高或孵育時(shí)間過(guò)長(zhǎng)

解決方法：降低抗體滴度、抗體孵育時(shí)間：室溫1小時(shí)或4度過(guò)夜

原 因：孵育溫度過(guò)高超過(guò)37度

解決方法：一般在室溫20-28度

原 因：DAB顯色時(shí)間過(guò)長(zhǎng)或濃度過(guò)高

解決方法：顯色時(shí)間不超過(guò)5-12分鐘，以顯微鏡下觀(guān)察為準(zhǔn)

2、非特異性背景染色？

原 因：操作過(guò)程中沖洗不充分

解決方法：每步?jīng)_洗3次每次5分鐘

原 因：組織中含過(guò)氧化物酶未阻斷

解決方法：可再配置新鮮3%H2O2封閉孵育時(shí)間延長(zhǎng)

原 因：組織中含內(nèi)原性*

解決方法：正常非免疫動(dòng)物血清再封閉

原 因：血清蛋白封閉不充分

解決方法：血清蛋白封閉不充分

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