上海研盟生物有限公司*品質(zhì)供應(yīng)Anti-A1CF抗體,我公司銷售國(guó)內(nèi)外科研產(chǎn)品,如生物試劑、生化試劑,一抗二抗,各種標(biāo)記抗體,Elisa酶聯(lián)免疫試劑盒,放免試劑盒,培養(yǎng)基,各種動(dòng)物血清等產(chǎn)品,歡迎廣大新老客戶前來咨詢訂購(gòu),訂購(gòu):或。
載脂蛋白B-mRNA編碼蛋白抗體,Anti-A1CF抗體簡(jiǎn)單介紹:
中文名稱:載脂蛋白B-mRNA編碼蛋白抗體
英文名稱:Anti-A1CF抗體
別 名:A1CF; A1CF_HUMAN; ACF; ACF64; ACF65; Apo B RNA editing protein; Apobec 1 complementation factor; Apobec 1 complementation factor (ACF) (ASP); APOBEC 1 stimulating protein; APOBEC1 complementation factor; APOBEC1 stimulating protein; APOBEC1-stimulating protein; APOBEC1CF; ASP; MGC163391; RP11-564C4.2.
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
背景介紹:Essential component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in APOB mRNA. Binds to APOB mRNA and is probably responsible for docking the catalytic subunit, APOBEC1, to the mRNA to allow it to deaminate its target cytosine. The complex also protects the edited APOB mRNA from nonsense-mediated decay.
規(guī)格:0.1ml/0.2ml
濃度:1mg/1ml
抗體來源:本公司抗體有兔來源和鼠來源等
克隆類型:兔來源為多抗,鼠來源為單抗
產(chǎn)品類型:一抗
載脂蛋白B-mRNA編碼蛋白抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
避免反復(fù)凍融,保質(zhì)期一年。
載脂蛋白B-mRNA編碼蛋白抗體,Anti-A1CF抗體免疫組化問題解答
1、染色過強(qiáng)?
原 因:抗體濃度過高或孵育時(shí)間過長(zhǎng)
解決方法:降低抗體滴度、抗體孵育時(shí)間:室溫1小時(shí)或4度過夜
原 因:孵育溫度過高超過37度
解決方法:一般在室溫20-28度
原 因:DAB顯色時(shí)間過長(zhǎng)或濃度過高
解決方法:顯色時(shí)間不超過5-12分鐘,以顯微鏡下觀察為準(zhǔn)
2、非特異性背景染色?
原 因:操作過程中沖洗不充分
解決方法:每步?jīng)_洗3次每次5分鐘
原 因:組織中含過氧化物酶未阻斷
解決方法:可再配置新鮮3%H2O2封閉孵育時(shí)間延長(zhǎng)
原 因:組織中含內(nèi)原性*
解決方法:正常非免疫動(dòng)物血清再封閉
原 因:血清蛋白封閉不充分
解決方法:血清蛋白封閉不充分
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