詳細(xì)介紹

概述
Sepax SEC鍵合固定相是采用的表面修飾技術(shù)，通過在高純度具有良好機(jī)械穩(wěn)定性的硅膠基質(zhì)上，鍵合一層均勻的納米厚度的中性親水涂層而制備得到。由于采用可控的化學(xué)修飾技術(shù)，因此能確保柱與柱之間有著可靠的重現(xiàn)性。SEC填料采用化學(xué)鍵合技術(shù)，表面親水涂層覆蓋*，因此不僅具有優(yōu)異的穩(wěn)定性，而且對蛋白等生物樣品的非特異性吸附作用也非常小。精心設(shè)計的大孔體積可保證高的分離容量以及優(yōu)異的分辨率。廣泛應(yīng)用于生物分子及水溶性聚合物的分離和檢測。

應(yīng)用領(lǐng)域

蛋白藥物（*、免疫球蛋白、HGH、EPO等）
多肽(Peptide)
水溶性多聚物（多糖、PEG）
抗生素（頭孢類抗生素）
抗體（Antibodies）、疫苗（Vaccine）
病毒蛋白(Virus Protein)、細(xì)胞發(fā)酵液、DNA

技術(shù)參數(shù)

固定相	SRT SEC-100	SRT SEC-150	SRT SEC-300	SRT SEC-500	SRT SEC-1000	SRT SEC-2000
材料	Neutral, hydrophilic film bonded silica	Neutral, hydrophilic film bonded silica	Neutral, hydrophilic film bonded silica	Neutral, hydrophilic film bonded silica	Neutral, hydrophilic film bonded silica	Neutral, hydrophilic film bonded silica
顆粒大小	5 mm	5 mm	5 mm	5 mm	5 mm	5 mm
孔徑 (Å)	~ 100	~ 150	~ 300	~ 500	~ 1,000	~ 2,000
蛋白分子量范圍	100 - 100,000	500 - 150,000	5,000 – 1,250,000	15,000 - 5,000,000	50,000 - 7,500,000	> 10,000,000
水溶性聚合物分子量范圍	500-10,000	500-25,000	1,000-100,000	2,500-500,000	5,000-1,500,000	50,000->2,500,000
pH穩(wěn)定性	2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.)	2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.)	2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.)	2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.)	2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.)	2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.)
反壓 (psi for a 7.8x300 mm)	~ 700	~ 700	~ 700	~700	~700	~700
壓力 (psi)	~ 4,500	~ 4,500	~ 3,500	~ 3,000	~ 3,000	~ 3,000
鹽濃度范圍	20 mM - 2.0 M	20 mM - 2.0 M	20 mM - 2.0 M	20 mM - 2.0 M	20 mM - 2.0 M	20 mM - 2.0 M
使用溫度	~ 80^oC	~ 80^oC	~ 80^oC	~ 80^oC	~ 80^oC	~ 80^oC
流動相兼容性	Aqueous and organic	Aqueous and organic	Aqueous and organic	Aqueous and organic	Aqueous and organic	Aqueous and organic

訂購信息

Sepax SRT SEC-100

產(chǎn)品名稱	粒度	孔徑	規(guī)格	訂貨號
SRT SEC-100	5um	100 Å	21.5x600mm	215100-21560
SRT SEC-100	5um	100 Å	21.5x300mm	215100-21530
SRT SEC-100	5um	100 Å	7.5x600mm	215100-7560
SRT SEC-100	5um	100 Å	7.8x300mm	215100-7830
SRT SEC-100	5um	100 Å	7.8x150mm	215100-7815
SRT SEC-100	5um	100 Å	7.8x50 (Guard)	215100-7805
SRT SEC-100	5um	100 Å	4.6x300mm	215100-4630
SRT SEC-100	5um	100 Å	4.6x150mm	215100-4615
SRT SEC-100	5um	100 Å	4.6x50 (Guard)	215100-4605

Sepax SRT SEC-150

產(chǎn)品名稱	粒度	孔徑	規(guī)格	訂貨號
SRT SEC-150	5um	150 Å	21.5x600mm	215150-21560
SRT SEC-150	5um	150 Å	21.5x300mm	215150-21530
SRT SEC-150	5um	150 Å	7.5x600mm	215150-7560
SRT SEC-150	5um	150 Å	7.8x300mm	215150-7830
SRT SEC-150	5um	150 Å	7.8x150mm	215150-7815
SRT SEC-150	5um	150 Å	7.8x50 (Guard)	215150-7805
SRT SEC-150	5um	150 Å	4.6x300mm	215150-4630
SRT SEC-150	5um	150 Å	4.6x150mm	215150-4615
SRT SEC-150	5um	150 Å	4.6x50 (Guard)	215150-4605

Sepax SRT SEC-300

產(chǎn)品名稱	粒度	孔徑	規(guī)格	訂貨號
SRT SEC-300	5um	300 Å	21.5x600mm	215300-21560
SRT SEC-300	5um	300 Å	21.5x300mm	215300-21530
SRT SEC-300	5um	300 Å	7.5x600mm	215300-7560
SRT SEC-300	5um	300 Å	7.8x300mm	215300-7830
SRT SEC-300	5um	300 Å	7.8x150mm	215300-7815
SRT SEC-300	5um	300 Å	7.8x50 (Guard)	215300-7805
SRT SEC-300	5um	300 Å	4.6x300mm	215300-4630
SRT SEC-300	5um	300 Å	4.6x150mm	215300-4615
SRT SEC-300	5um	300 Å	4.6x50 (Guard)	215300-4605

Sepax SRT SEC-500

產(chǎn)品名稱	粒度	孔徑	規(guī)格	訂貨號
SRT SEC-500	5um	500 Å	21.5x600mm	215500-21560
SRT SEC-500	5um	500 Å	21.5x300mm	215500-21530
SRT SEC-500	5um	500 Å	7.5x600mm	215500-7560
SRT SEC-500	5um	500 Å	7.8x300mm	215500-7830
SRT SEC-500	5um	500 Å	7.8x150mm	215500-7815
SRT SEC-500	5um	500 Å	7.8x50 (Guard)	215500-7805
SRT SEC-500	5um	500 Å	4.6x300mm	215500-4630
SRT SEC-500	5um	500 Å	4.6x150mm	215500-4615
SRT SEC-500	5um	500 Å	4.6x50 (Guard)	215500-4605

Sepax SRT SEC-1000

產(chǎn)品名稱	粒度	孔徑	規(guī)格	訂貨號
SRT SEC-1000	5um	1000 Å	21.5x600mm	215950-21560
SRT SEC-1000	5um	1000 Å	21.5x300mm	215950-21530
SRT SEC-1000	5um	1000 Å	7.5x600mm	215950-7560
SRT SEC-1000	5um	1000 Å	7.8x300mm	215950-7830
SRT SEC-1000	5um	1000 Å	7.8x150mm	215950-7815
SRT SEC-1000	5um	1000 Å	7.8x50 (Guard)	215950-7805
SRT SEC-1000	5um	1000 Å	4.6x300mm	215950-4630
SRT SEC-1000	5um	1000 Å	4.6x150mm	215950-4615
SRT SEC-1000	5um	1000 Å	4.6x50 (Guard)	215950-4605

Sepax SRT SEC-2000

產(chǎn)品名稱	粒度	孔徑	規(guī)格	訂貨號
SRT SEC-2000	5um	2000 Å	21.5x600mm	215980-21560
SRT SEC-2000	5um	2000 Å	21.5x300mm	215980-21530
SRT SEC-2000	5um	2000 Å	7.5x600mm	215980-7560
SRT SEC-2000	5um	2000 Å	7.8x300mm	215980-7830
SRT SEC-2000	5um	2000 Å	7.8x150mm	215980-7815
SRT SEC-2000	5um	2000 Å	7.8x50 (Guard)	215980-7805
SRT SEC-2000	5um	2000 Å	4.6x300mm	215980-4630
SRT SEC-2000	5um	2000 Å	4.6x150mm	215980-4615
SRT SEC-2000	5um	2000 Å	4.6x50 (Guard)	215980-4605