huh7細(xì)胞
細(xì)胞名稱(chēng):huh7細(xì)胞 (STR鑒定正確)
細(xì)胞別稱(chēng): HuH-7; HUH-7; HuH7; Huh7; HUH7; HUH7.0; JTC-39; Japanese Tissue Culture-39
貨號(hào):TCH-C217
規(guī)格: 1X10^6
價(jià)格:1500元 (干冰運(yùn)輸需另加200元干冰費(fèi))
種屬來(lái)源:人
組織來(lái)源:肝臟
特征:肝癌
細(xì)胞形態(tài):上皮細(xì)胞樣
生長(zhǎng)特性:貼壁生長(zhǎng)
培養(yǎng)體系:DMEM-H+10%FBS+1%Glutamax+1% Sodium Pyruvate+1%P/S
huh7細(xì)胞培養(yǎng)基貨號(hào):TCH-G217
傳代比例:1:2-1:3,每2-3天換液一次
傳代周期:48-72 h
培養(yǎng)條件:氣相:95%空氣+5%CO2,溫度:37℃
凍存條件:60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO,液氮儲(chǔ)存
質(zhì)量檢測(cè):細(xì)菌、真菌、支原體檢測(cè)均為陰性
huh-7細(xì)胞 產(chǎn)甲胎蛋白,α抗體,血漿銅藍(lán)蛋白,纖維蛋白原,纖維粘連蛋白等。
參考文獻(xiàn):
Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)
Kasai F., Hirayama N., Ozawa M., Satoh M., Kohara A.
HuH-7 reference genome profile: complex karyotype composed of massive loss of heterozygosity.
Hum. Cell 31:261-267(2018)
Shi J., Wang X., Lyu L., Jiang H., Zhu H.-J.
Comparison of protein expression between human livers and the hepatic cell lines HepG2, Hep3B, and Huh7 using SWATH and MRM-HR proteomics: Focusing on drug-metabolizing enzymes.
Drug Metab. Pharmacokinet. 33:133-140(2018)
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