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Applications:
transfection host （Nucleofection technology from Lonza Roche FuGENE® Transfection Reagents）
This line is a variant of the T1 cell line （ATCC CRL-1991） produced by selection the SFR1-MI.3 monoclonal antibody （against a monomorphic determinant on HLA DR）. The cells do not express HLA DR and are Class II major histocompatibility （MHC） antigen negative. The cells synthesize, but do not express, HLA B5. T2 appears to have lost both copies of the CEMR.3 derived chromosome 6. Together the T1 and T2 lines are useful for studying antigen processing and T cell recognition of class I MHC antigens.
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. Temperature: 37.0°C
Subculturing:
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 X 10（5） viable cells/ml and maintain between 3 X 10（5） and 1 X 10（6） viable cells/ml. Medium Renewal: Every 2 to 3 days
Preservation:
Freeze medium: Complete growth medium supplemented with 5% （v/v） DMSO Storage temperature: liquid nitrogen vapor phase
Related Products:
Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2005 recommended serum:ATCC 30-2020
References:
22647: Salter RD, et al. Genes regulating HLA class I antigen expression in T-B lymphoblast hybrids. Immunogenetics 21: 235-246, 1985. PubMed: 3872841
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