人B-細(xì)胞激活因子（BAFF）檢測試劑盒全國：歡迎選購！,或咨詢?cè)诰€客服,或者以郵件形式發(fā)到我司741653262，公司提供*進(jìn)口，國產(chǎn)elisa試劑盒，均為高性價(jià)比試劑盒

產(chǎn)品名稱：人B-細(xì)胞激活因子(BAFF) ELISA Kit
規(guī)格：96T/48T
檢測標(biāo)本：血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等
檢測方法：ELISA
檢測類型：酶聯(lián)免疫夾心法
運(yùn)輸保存：2~8°保存6個(gè)月
人B-細(xì)胞激活因子（BAFF）檢測試劑盒齊一生物主要經(jīng)營進(jìn)口原裝Elisa試劑盒全國：,種類齊全,價(jià)格實(shí)惠,購買齊一生物產(chǎn)品的新老客戶,可享免費(fèi)代測實(shí)驗(yàn)服務(wù),可檢測人源,小鼠,大鼠,豬,狗,猴,兔,羊,雞,牛,鴨子,魚,植物等各種樣本.全程提供.如需其他Elisa試劑盒說明書及報(bào)價(jià),可齊一生物.
特點(diǎn)
一、高效、靈敏、特異的抗體；
二、穩(wěn)定的重復(fù)性和可靠性；
三、吸附性能好,空白值低,孔底透明度高的固相載體；
四、適用血清、血漿、組織勻漿液、細(xì)胞培養(yǎng)上清液、尿液等等多種標(biāo)本類型；
五、節(jié)省實(shí)驗(yàn)經(jīng)費(fèi).
elisa試劑盒回收率是反應(yīng)待測物在樣品分析過程中的損失的程度,損失越少,回收率越高,如果作標(biāo)液1PPM,就是1毫克/升,而作出標(biāo)準(zhǔn)數(shù)據(jù)為0.99毫克/升,就是說你的回收率是99%,這個(gè)與真實(shí)成分有密切的關(guān)系,說明方法的準(zhǔn)確度.比如水中總無機(jī)氯含量測定,樣品水中含有無機(jī)氯20mg/L,取100mL被測水樣品,加入0.1mL濃度為10mg/mL的含無機(jī)氯標(biāo)準(zhǔn)樣品,測定時(shí)忽略體積變化,如果測定出樣品中無機(jī)氯為2.98mg/L,則認(rèn)為回收率為99%.
制備方法
包括以下步驟：
1)抗原表位的計(jì)算機(jī)篩選；
2)抗原的制備；
3)血清的采集；
4)間接ELISA方法的建立；
5)間接ELISA方法的敏感性和特異性驗(yàn)證；
6)試劑盒的穩(wěn)定性驗(yàn)證；
7)對(duì)屠宰場進(jìn)行臨床檢測.該方法簡單、快速、靈敏度高、特異性強(qiáng)、穩(wěn)定性合格、重復(fù)性好.應(yīng)用本發(fā)明制得的試劑盒能有效檢出感染豬的戊型肝炎病毒,適用于大批量發(fā)病樣本的檢測,可用于豬戊肝的快速診斷,并預(yù)防、控制豬戊肝病毒的流行和阻斷戊肝病毒由豬向人傳播.
組成結(jié)構(gòu)
1、血清：操作過程中避免任何細(xì)胞刺激.使用不含熱原和內(nèi)毒素的試管.收集血液后,1000×g離心10分鐘將血紅細(xì)胞迅速小心地分離.
2、血漿：EDTA、檸檬酸鹽、肝素血漿可用于檢測.1000×g離心30分鐘去除顆粒.
3、細(xì)胞上清液：1000×g離心10分鐘去除顆粒和聚合物.
4、組織勻漿：將組織加入適量生理鹽水搗碎.1000×g離心10分鐘,取上清液.
5、保存：如果樣品不立即使用,應(yīng)將其分成小部分-70℃保存,避免反復(fù)冷凍.盡可能的不要使用溶血或高血脂血.如果血清中大量顆粒,檢測前先離心或過濾.不要在37℃或更高的溫度加熱解凍.應(yīng)在室溫下解凍并確保樣品均勻地充分解凍
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人肺癌細(xì)胞 PC-9（PC9）
人轉(zhuǎn)移胰腺癌細(xì)胞 AsPC-1
人急性非B非T淋巴細(xì)胞白血病細(xì)胞 Reh
人肺癌細(xì)胞 MSTO-211H
人肺成纖維樣細(xì)胞 HLF
人B淋巴瘤細(xì)胞 Farage
人肺癌細(xì)胞 NCI-H596
人肺鱗癌細(xì)胞 SK-MES-1
人結(jié)腸癌細(xì)胞 RKO
人鼻咽癌細(xì)胞 SUNE-1亞株-6-10B
人胚腎細(xì)胞 293A
人神經(jīng)母細(xì)胞瘤細(xì)胞 SK-N-SH
人非小細(xì)胞肺癌細(xì)胞 NCI-H358
人肺腺癌細(xì)胞 NCI-H1975
人巨核細(xì)胞白血病細(xì)胞 DAMI
人支氣管上皮細(xì)胞 HBE
人膠質(zhì)母細(xì)胞瘤細(xì)胞 A172
人膀胱移行細(xì)胞乳頭瘤細(xì)胞 RT4
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大鼠雪旺細(xì)胞 RSC96
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