人肌抑素（MSTN）ELISA檢測試劑盒?檢測方法：酶聯(lián)免疫分析ELISA方法
?有效期： 4 個月(4℃)；8 個月（-20℃）。 ?保存方法： 2-8℃（頻繁使用時）; -20℃（長時間不用時）。 ?品牌：子科生物ZIKER,美國R&D,美國immonoway,美國sciencell,德國IBL,
?質(zhì)量保證：我告訴所有產(chǎn)品均需要經(jīng)過質(zhì)檢通過后，才允許出庫！?適用范圍：實驗結(jié)果僅供科研參考，不推薦用于臨床診斷結(jié)果。?ELISA常用的方法：雙抗體夾心法、間接法、競爭法以及BAS-ELISA等，子科生物ELISA產(chǎn)品主要用雙抗體夾心法。?標(biāo)本：血清、血漿、細(xì)胞上清液、尿液、體液、灌洗液、腦脊髓、心房水、胸房水、組織等?產(chǎn)品性能：靈敏度高，特異性強，重復(fù)性。

人肌抑素（MSTN）ELISA檢測試劑盒Assay procedure
1.  Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.
2.  Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.
3.  Add Sample: Add testing sample 10μl then add Sample Diluent 40μl to testing sample well; Blank well doesn’t add anyting.
4.  Add 100μl of HRP-conjugate reagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37°C. 
5.  Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels.
6.  Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.
7.  Add 50μl Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not 
appear uniform, gently tap the plate to ensure thorough mixing.
8.  Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes.
Calculation of results
1.This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis. 
2.First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software. 
3.To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration. 
4.Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.
5.The sensitivity by this assay is 10.0 pg/ml
6.Standard curve
Storage：  2-8℃.
validity： six months.

ZK-H2569    人核仁磷酸蛋白(NPM)ELISA檢測試劑盒    human Nucleophosmin (NPM) ELISA Kit    ELISA.     96T/48T    ZIKER子科生物
ZK-H2570    人抑絲蛋白1(PFN1)ELISA檢測試劑盒    human Profilin 1 (PFN1) ELISA Kit    ELISA.     96T/48T    ZIKER子科生物
ZK-H2571    人核仁素(NCL)ELISA檢測試劑盒    human Nucleolin (NCL) ELISA Kit    ELISA.     96T/48T    ZIKER子科生物
ZK-H2572    人染色框同源物3(CBX3)ELISA檢測試劑盒    human Chromobox Homolog 3 (CBX3) ELISA Kit    ELISA.     96T/48T    ZIKER子科生物
ZK-H2573    人核糖體蛋白L6(RPL6)ELISA檢測試劑盒    human Ribosomal Protein L6 (RPL6) ELISA Kit    ELISA.     96T/48T    ZIKER子科生物
ZK-H2574    人肝細(xì)胞癌相關(guān)蛋白1(HCRP1)ELISA檢測試劑盒    human Hepatocellular Carcinoma Related Protein 1 (HCRP1) ELISA Kit    ELISA.     96T/48T    ZIKER子科生物
ZK-H2575    人瘤變抑制因子14(ST14)ELISA檢測試劑盒    human Suppression Of Tumorigenicity 14 (ST14) ELISA Kit    ELISA.     96T/48T    ZIKER子科生物
ZK-H2576    人透明質(zhì)酸介導(dǎo)運動因子受體(HMMR)ELISA檢測試劑盒    human Hyaluronan Mediated Motility Receptor (HMMR) ELISA Kit    ELISA.     96T/48T    ZIKER子科生物
ZK-H2577    人血管生成素樣蛋白6(ANGPTL6)ELISA檢測試劑盒    human Angiopoietin Like Protein 6 (ANGPTL6) ELISA Kit    ELISA.     96T/48T    ZIKER子科生物
ZK-H2578    人封閉蛋白9(CLDN9)ELISA檢測試劑盒    human Claudin 9 (CLDN9) ELISA Kit    ELISA.     96T/48T    ZIKER子科生物
ZK-H2579    人轉(zhuǎn)酮醇酶(TKT)ELISA檢測試劑盒    human Transketolase (TKT) ELISA Kit    ELISA.     96T/48T    ZIKER子科生物
ZK-H2580    人含堿性螺旋環(huán)螺旋域蛋白B8(BHLHB8)ELISA檢測試劑盒    human ZIKERsic Helix Loop Helix Domain Containing Protein B8 (BHLHB8) ELISA Kit    ELISA.     96T/48T    ZIKER子科生物
ZK-H2581    人Duffy血型趨化因子受體(DARC)ELISA檢測試劑盒    human Duffy Blood Group Chemokine Receptor (DARC) ELISA Kit    ELISA.     96T/48T    ZIKER子科生物
ZK-H2582    人X-框結(jié)合蛋白1(XBP1)ELISA檢測試劑盒    human X-Box Binding Protein 1 (XBP1) ELISA Kit    ELISA.     96T/48T    ZIKER子科生物
ZK-H2583    人牛痘相關(guān)激酶1(VRK1)ELISA檢測試劑盒    human Vaccinia Related Kinase 1 (VRK1) ELISA Kit    ELISA.     96T/48T    ZIKER子科生物
ZK-H2584    人細(xì)胞色素P450家族成員24A1(CYP24A1)ELISA檢測試劑盒    human Cytochrome P450 24A1 (CYP24A1) ELISA Kit    ELISA.     96T/48T    ZIKER子科生物
ZK-H2585    人質(zhì)膜膜泡關(guān)聯(lián)蛋白(PLVAP)ELISA檢測試劑盒    human Plasmalemma Vesicle Associated Protein (PLVAP) ELISA Kit    ELISA.     96T/48T    ZIKER子科生物
ZK-H2586    人Toll樣受體6(TLR6)ELISA檢測試劑盒    human Toll Like Receptor 6 (TLR6) ELISA Kit    ELISA.     96T/48T    ZIKER子科生物
ZK-H2587    人丙糖磷酸異構(gòu)酶1(TPI1)ELISA檢測試劑盒    human Triosephosphate Isomerase 1 (TPI1) ELISA Kit    ELISA.     96T/48T    ZIKER子科生物
ZK-H2588    人同種異體移植炎癥因子1(AIF1)ELISA檢測試劑盒    human Allograft Inflammatory Factor 1 (AIF1) ELISA Kit    ELISA.     96T/48T    ZIKER子科生物
ZK-H2589    人分揀蛋白1(SORT1)ELISA檢測試劑盒    human Sortilin 1 (SORT1) ELISA Kit    ELISA.     96T/48T    ZIKER子科生物
ZK-H2590    人淀粉樣蛋白β前體樣蛋白2(APLP2)ELISA檢測試劑盒    human Amyloid Beta Precursor Like Protein 2 (APLP2) ELISA Kit    ELISA.     96T/48T    ZIKER子科生物
ZK-H2591    人鈣離子轉(zhuǎn)運ATP酶A2(ATP2A2)ELISA檢測試劑盒    human ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) ELISA Kit    ELISA.     96T/48T    ZIKER子科生物
ZK-H2592    人賴氨酰氧化酶(LOX)ELISA檢測試劑盒    human Lysyl Oxidase (LOX) ELISA Kit    ELISA.     96T/48T    ZIKER子科生物
ZK-H2593    人蛋氨酸腺苷轉(zhuǎn)移酶Ⅱα(MAT2α)ELISA檢測試劑盒    human Methionine Adenosyltransferase II Alpha (MAT2a) ELISA Kit    ELISA.     96T/48T    ZIKER子科生物
ZK-H2594    人GPI錨定分子樣蛋白(GML)ELISA檢測試劑盒    human Glycosylphosphatidylinositol Anchored Molecule Like Protein (GML) ELISA Kit    ELISA.     96T/48T    ZIKER子科生物
ZK-H2595    人核糖體蛋白L23A(RPL23A)ELISA檢測試劑盒    human Ribosomal Protein L23A (RPL23A) ELISA Kit    ELISA.     96T/48T    ZIKER子科生物
ZK-H2596    人同線蛋白2(ST2)ELISA檢測試劑盒    human Syntenin 2 (ST2) ELISA Kit    ELISA.     96T/48T    ZIKER子科生物
ZK-H2597    人白介素1家族成員9(IL1F9)ELISA檢測試劑盒    human Interleukin 1 Family, Member 9 (IL1F9) ELISA Kit    ELISA.     96T/48T    ZIKER子科生物
ZK-H2598    人鉀中間小電導(dǎo)鈣激活通道蛋白亞家族N成員2(KCNN2)ELISA檢測試劑盒    human Potassium Intermediate Small Conductance Calcium Activated Channel Subfamily N, Member 2 (KCNN2) ELISA Kit    ELISA.     96T/48T    ZIKER子科生物
ZK-H2599    人增殖關(guān)聯(lián)蛋白2G4(PA2G4)ELISA檢測試劑盒    human Proliferation Associated Protein 2G4 (PA2G4) ELISA Kit    ELISA.     96T/48T    ZIKER子科生物
ZK-H2600    人膜聯(lián)蛋白A4(ANXA4)ELISA檢測試劑盒    human Annexin A4 (ANXA4) ELISA Kit    ELISA.     96T/48T    ZIKER子科生物