人脂氧素A4(LXA4)ELISA檢測(cè)試劑盒
●規(guī)格: 96T/48T
●品牌:子科生物ZIKER
●反應(yīng)時(shí)間: 1-5h
●所需樣本體積: 50-100ul
●檢測(cè)波長(zhǎng): 450 nm
●檢測(cè)范圍:歡迎2811391951或電蘇索取原版說(shuō)明書(shū)
人脂氧素A4(LXA4)ELISA檢測(cè)試劑盒
[保存條件]保質(zhì)期2-8℃低溫保存下,6個(gè)月的保質(zhì)期。
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人脂氧素A4(LXA4)ELISA檢測(cè)試劑盒組成:結(jié)果判定常用的幾種方法
1) 目測(cè)定性法:加入底物經(jīng)酶解和終止反應(yīng)后,肉眼觀察陽(yáng)性孔顏色明顯深于(競(jìng)爭(zhēng)法則淺于)陰性對(duì)照;與陰性對(duì)照的顏色接近者,判定為陰性。
2) 以樣品孔的OD值大于陰性對(duì)照OD值+2~3SD,作為陽(yáng)性結(jié)果的閾值。
3) 以P/N值(陽(yáng)性孔OD值/陰性孔OD值)大于或等于2.1為陽(yáng)性;P/N值小于2.1,但大于1.5為可疑;P/N小于1.5為陰性。
定量測(cè)定結(jié)果根據(jù)標(biāo)準(zhǔn)曲線計(jì)算樣品中待測(cè)物的含量。
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