人組織因子(TF)ELISA檢測(cè)試劑盒
?品牌:子科生物ZIKER,美國(guó)R&D,美國(guó)immonoway,美國(guó)sciencell,德國(guó)IBL
?有效期: 4 個(gè)月(4℃);8 個(gè)月(-20℃)。
?保存方法: 2-8℃(頻繁使用時(shí)); -20℃(長(zhǎng)時(shí)間不用時(shí))。
?服務(wù):子科生物所有的elisa試劑盒均可免費(fèi)代測(cè),全程Elisa實(shí)驗(yàn)技術(shù)指導(dǎo)
人組織因子(TF)ELISA檢測(cè)試劑盒子科生物免費(fèi)代測(cè),2016年年中*!進(jìn)行詢價(jià),全場(chǎng)可以享受7折優(yōu)惠!并可享受滿減活動(dòng),滿10000減1000,滿5000減200元,滿1000元減50元,期待與您的合作!
人組織因子(TF)ELISA檢測(cè)試劑盒樣品收集、處理及保存方法:
1、 血清……操作過程中避免任何細(xì)胞刺激。使用不含熱原和內(nèi)毒素的試管。收集血液后,1000×g離心10分鐘將血紅細(xì)胞迅速小心地分離。
2、 血漿……EDTA、檸檬酸鹽、肝素血漿可用于檢測(cè)。1000×g離心30分鐘去除顆粒。
3、 細(xì)胞上清液……1000×g離心10分鐘去除顆粒和聚合物。
4、 組織勻漿……將組織加入適量理鹽水搗碎。1000×g離心10分鐘,取上清液。
5、 保存……如果樣品不立即使用,應(yīng)將其分成小部分-70℃保存,避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒,檢測(cè)前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。
試劑的準(zhǔn)備:
1. 標(biāo)準(zhǔn)品:標(biāo)準(zhǔn)品的系列稀釋應(yīng)在實(shí)驗(yàn)時(shí)準(zhǔn)備,不能儲(chǔ)存。稀釋前將標(biāo)準(zhǔn)品振蕩混勻。稀釋比例按下表中進(jìn)行:
500pg/ml(6號(hào)標(biāo)準(zhǔn)品) 原倍濃度不用稀釋直接加入50ul
250pg/ml(5號(hào)標(biāo)準(zhǔn)品) 100ul的原倍標(biāo)準(zhǔn)品加入100ul的標(biāo)準(zhǔn)品稀釋液
125pg/ml(4號(hào)標(biāo)準(zhǔn)品) 100ul的5號(hào)標(biāo)準(zhǔn)品加入100ul的標(biāo)準(zhǔn)品稀釋液
62.5pg/ml(3號(hào)標(biāo)準(zhǔn)品) 100ul的4號(hào)標(biāo)準(zhǔn)品加入100ul的標(biāo)準(zhǔn)品稀釋液
31.2pg/ml(2號(hào)標(biāo)準(zhǔn)品) 100ul的3號(hào)標(biāo)準(zhǔn)品加入100ul的標(biāo)準(zhǔn)品稀釋液
15.6pg/ml(1號(hào)標(biāo)準(zhǔn)品) 100ul的2號(hào)標(biāo)準(zhǔn)品加入100ul的標(biāo)準(zhǔn)品稀釋液
0pg/ml (空白對(duì)照) 原倍濃度不用稀釋直接加入50ul
2. 洗滌緩沖液(50×)的稀釋:蒸餾水50倍稀釋。
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