人鳥苷酸解離抑制因子(GDI)ELISA檢測試劑盒
?標(biāo)本:血清、血漿、細(xì)胞上清液、尿液、體液、灌洗液、腦脊髓、心房水、胸房水、組織等
?產(chǎn)品性能:靈敏度高,特異性強,重復(fù)性。
?產(chǎn)品包裝:盒(KIT)(含試劑+酶聯(lián)板+覆膜等)
?檢測范圍:詳細(xì)請咨詢在線客服2811391951,或銷售蘇。
人鳥苷酸解離抑制因子(GDI)ELISA檢測試劑盒子科生物研發(fā)生產(chǎn)現(xiàn)貨供應(yīng),深圳子科生物提供大量品牌進(jìn)口原裝,分裝,以及穩(wěn)定性強的自產(chǎn)國產(chǎn)人ELISA試劑盒,大鼠ELISA試劑盒,小鼠ELISA試劑盒,魚ELISA試劑盒,植物ELISA試劑盒,牛羊豬雞ELISA試劑盒性價比*。咨詢!
人鳥苷酸解離抑制因子(GDI)ELISA檢測試劑盒就是將抗原抗體固定到特定的載體上進(jìn)行反應(yīng),并通過酶催化底物發(fā)生化學(xué)變化將抗原抗體反應(yīng)通過顏色的形式顯現(xiàn)出來。
(1)將抗原抗體反應(yīng)固相化:即試驗中的包被環(huán)節(jié),使抗原或抗體吸附于固相載體表面。其機制可能是聚苯乙烯表面間的疏水基團可以無選擇性地吸附蛋白分子。此物理性吸附不破壞蛋白分子結(jié)構(gòu),保持其生物學(xué)特性和免疫學(xué)活性;
(2)封閉:固相吸附蛋白是非特異性的,在包被目的免疫蛋白后,未吸附蛋白的固相表面仍然有吸附其它蛋白的能力,為了保障抗原抗體反應(yīng)的特異性,因此,包被剩余的固相表面應(yīng)該用抗原抗體反應(yīng)無關(guān)蛋白封閉。一般認(rèn)為牛血清白蛋白是zui為理想的封閉劑,也可用脫脂奶粉、明膠、牛血清替代。
(3)抗原抗體反應(yīng):抗原或抗體在適當(dāng)?shù)慕橘|(zhì)下可進(jìn)行特異的抗原抗體反應(yīng)。 (4)酶反應(yīng):酶可以通過共價鍵與抗原或抗體連接形成結(jié)合物,當(dāng)抗原抗體反應(yīng)的時候,被檢測靶目標(biāo)中也結(jié)合了酶分子。
(5)ELISA試劑盒底物反應(yīng):結(jié)合在抗原抗體中的酶分子,可以促使底物發(fā)生顏色反應(yīng),檢測人員可以裸眼觀察,可以通過顏色來判定是否有免疫反應(yīng)的存在,通過顏色的深淺判斷標(biāo)本中相應(yīng)抗原或抗體的含量,也可借用酶標(biāo)儀在適當(dāng)?shù)牟ㄩL讀取吸光度值。
實驗所涉及到的儀器如下:酶標(biāo)儀、洗板機、離心機、培養(yǎng)箱
小鼠半乳凝素8(GAL8) ELISA Kit 96T 48T 0.156-10ng/mL 0.055ng/mL mouse Galectin 8 (GAL8) ELISA Kit Mus musculus mouse
豬半乳凝素8(GAL8) ELISA Kit 96T 48T 0.313-20 ng/mL 0.062 ng/mL porcine Galectin 8 (GAL8) ELISA Kit Sus scrofa; Porcine Pig
大鼠半乳凝素8(GAL8) ELISA Kit 96T 48T 0.156-10 ng/mL 0.028 ng/mL Rat Galectin 8 (GAL8) ELISA Kit Rattus norvegicus Rat
豬胰蛋白酶(TRY) ELISA Kit 96T 48T 1.563-100ng/mL 0.58ng/mL porcine Trypsin (TRY) ELISA Kit Sus scrofa; Porcine Pig
人雙腎上腺皮質(zhì)激素(DCX) ELISA Kit 96T 48T 0.156-10ng/mL 0.058ng/mL human Doublecortin (DCX) ELISA Kit Homo sapiens human
人Ⅲ型膠原α1(COL3α1) ELISA Kit 96T 48T 0.625-40ng/mL 0.226ng/mL human Collagen Type III Alpha 1 (COL3a1) ELISA Kit Homo sapiens human
人半乳凝素12(GAL12) ELISA Kit 96T 48T 0.313-20 ng/mL 0.085 ng/mL human Galectin 12 (GAL12) ELISA Kit Homo sapiens human
小鼠半乳凝素12(GAL12) ELISA Kit 96T 48T 31.25-2000pg/mL 14.5pg/mL mouse Galectin 12 (GAL12) ELISA Kit Mus musculus mouse
小鼠半乳凝素6(GAL6) ELISA Kit 96T 48T 0.156-10 ng/mL 0.034 ng/mL mouse Galectin 6 (GAL6) ELISA Kit Mus musculus mouse
牛半乳凝素1(GAL1) ELISA Kit 96T 48T 78.125-5000pg/mL 27.5pg/mL Bovine Galectin 1 (GAL1) ELISA Kit Bos taurus; Bovine Cattle
山羊半乳凝素1(GAL1) ELISA Kit 96T 48T 0.625-40 ng/mL 0.109 ng/mL Goat Galectin 1 (GAL1) ELISA Kit Capra hircus; Caprine Goat
人半乳凝素1(GAL1) ELISA Kit 96T 48T 0.781-50ng/mL 0.29ng/mL human Galectin 1 (GAL1) ELISA Kit Homo sapiens human
小鼠半乳凝素1(GAL1) ELISA Kit 96T 48T 0.781-50ng/mL 0.31ng/mL mouse Galectin 1 (GAL1) ELISA Kit Mus musculus mouse
豬半乳凝素1(GAL1) ELISA Kit 96T 48T 0.625-40 ng/mL 0.125 ng/mL porcine Galectin 1 (GAL1) ELISA Kit Sus scrofa; Porcine Pig
大鼠半乳凝素1(GAL1) ELISA Kit 96T 48T 0.625-40ng/mL 0.246ng/mL Rat Galectin 1 (GAL1) ELISA Kit Rattus norvegicus Rat
人基質(zhì)金屬蛋白酶15(MMP15) ELISA Kit 96T 48T 78.125-5000pg/mL 25pg/mL human Matrix Metalloproteinase 15 (MMP15) ELISA Kit Homo sapiens human
小鼠基質(zhì)金屬蛋白酶15(MMP15) ELISA Kit 96T 48T 78.125-5000pg/mL 28.4pg/mL mouse Matrix Metalloproteinase 15 (MMP15) ELISA Kit Mus musculus mouse
豬基質(zhì)金屬蛋白酶15(MMP15) ELISA Kit 96T 48T 0.313-20 ng/mL 0.094 ng/mL porcine Matrix Metalloproteinase 15 (MMP15) ELISA Kit Sus scrofa; Porcine Pig
人S100鈣結(jié)合蛋白(S100) ELISA Kit 96T 48T 0.313-20ng/mL 0.123ng/mL human S100 Calcium Binding Protein (S100) ELISA Kit Homo sapiens human
人角蛋白1(KRT1) ELISA Kit 96T 48T 0.156-10ng/mL 0.061ng/mL human Keratin 1 (KRT1) ELISA Kit Homo sapiens human
人胰島素降解酶(IDE) ELISA Kit 96T 48T 62.5-4000pg/mL 22.7pg/mL human Insulin Degrading Enzyme (IDE) ELISA Kit Homo sapiens human
人Lacritin蛋白(LACRT) ELISA Kit 96T 48T 0.156-10ng/mL 0.055ng/mL human Lacritin (LACRT) ELISA Kit Homo sapiens human
人皮敵菌素(DCD) ELISA Kit 96T 48T 0.781-50ng/mL 0.31ng/mL human Dermcidin (DCD) ELISA Kit Homo sapiens human
山羊半乳凝素14(GAL14) ELISA Kit 96T 48T 0.156-10 ng/mL 0.052 ng/mL Goat Galectin 14 (GAL14) ELISA Kit Capra hircus; Caprine Goat
人半乳凝素14(GAL14) ELISA Kit 96T 48T 0.313-20 ng/mL 0.15 ng/mL human Galectin 14 (GAL14) ELISA Kit Homo sapiens human
猴半乳凝素14(GAL14) ELISA Kit 96T 48T 0.156-10 ng/mL 0.048 ng/mL Monkey Galectin 14 (GAL14) ELISA Kit Rhesus monkey Simian
小鼠腎足蛋白(NPHN) ELISA Kit 96T 48T 0.313-20ng/mL 0.123ng/mL mouse Nephrin (NPHN) ELISA Kit Mus musculus mouse
豬肌鈣蛋白T(TNT) ELISA Kit 96T 48T 15.625-1000pg/mL 6.4pg/mL porcine Troponin T (TNT) ELISA Kit Sus scrofa; Porcine Pig