人結(jié)合珠蛋白/觸珠蛋白(Hpt/HP)ELISA檢測試劑盒子科生物免費(fèi)代測,2016年年中*!進(jìn)行詢價(jià),全場可以享受7折優(yōu)惠!并可享受滿減活動(dòng),滿10000減1000,滿5000減200元,滿1000元減50元,期待與您的合作!
人結(jié)合珠蛋白/觸珠蛋白(Hpt/HP)ELISA檢測試劑盒
?產(chǎn)品規(guī)格:96T(人份)/48T(人份)
單孔檢測:96T,可以檢測90份樣本,留5個(gè)孔做標(biāo)準(zhǔn)曲線,一個(gè)孔做空白對照;
48T,可以檢測42份樣本,留5個(gè)孔做標(biāo)準(zhǔn)曲線,一個(gè)孔做空白對照;
?產(chǎn)品包裝:盒(KIT)(含試劑+酶聯(lián)板+覆膜等)
?適用范圍:實(shí)驗(yàn)結(jié)果僅供科研參考,不推薦用于臨床診斷結(jié)果。
?有效期: 4 個(gè)月(4℃);8 個(gè)月(-20℃)。
●人結(jié)合珠蛋白/觸珠蛋白(Hpt/HP)ELISA檢測試劑盒標(biāo)本的采集及保存:
1.血清:標(biāo)本請于室溫放置2小時(shí)或4℃過夜后于1000 x g離心20分鐘,取上清即可檢測,或?qū)?biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融。
2.血漿:可用EDTA或肝素作為抗凝劑,標(biāo)本采集后30分鐘內(nèi)于2 - 8° C 1000 x g離心15分鐘,或?qū)?biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融。
●操作步驟
各試劑在使用前平衡至室溫。
1. 加樣:分別設(shè)空白孔、標(biāo)準(zhǔn)孔、待測樣品孔。除空白孔外,余孔分別加標(biāo)準(zhǔn)溶液或待測樣品100ul,注意不要有氣泡,輕輕混勻,酶標(biāo)板加上蓋,37℃反應(yīng)120分鐘。
2. 棄去液體,甩干,不用洗滌。
3. 每孔加檢測溶液A工作液 100ul,37℃,60分鐘。洗板3次,350ul/每孔,甩干。
4. 每孔加檢測溶液B工作液 100ul,37℃,60分鐘,洗板5次,甩干。
5. 依序每孔加底物溶液90ul,37℃避光顯色30分鐘(此時(shí)肉眼可見標(biāo)準(zhǔn)品的前3-4孔有明顯的梯度蘭色,后3-4孔梯度不明顯)。
6. 依序每孔加終止溶液50ul,終止反應(yīng)(此時(shí)蘭色立轉(zhuǎn)黃色)。用酶聯(lián)儀在450nm波長依序測量各孔的光密度(OD值)。
●注意:1.每次實(shí)驗(yàn)留一孔作為空白調(diào)零孔,該孔不加任何試劑,只是zui后加底物溶液及2NH2SO4。測量時(shí)先用此孔調(diào)OD值至零。
2.為防止樣品蒸發(fā),試驗(yàn)時(shí)將反應(yīng)板放于鋪有濕布的密閉盒內(nèi)。
●洗板方法:
手工洗板方法:吸去(不可觸及板壁)或甩掉酶標(biāo)板內(nèi)的液體;在實(shí)驗(yàn)臺(tái)上鋪墊幾層吸水紙,酶標(biāo)板朝下用力拍幾次;將推薦的洗滌緩沖液至少0.4ml注入孔內(nèi),浸泡1-2分鐘,根據(jù)需要,重復(fù)此過程數(shù)次。
自動(dòng)洗板:如果有自動(dòng)洗板機(jī),應(yīng)在熟練使用后再用到正式實(shí)驗(yàn)過程中。
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