人胰島素原(PI)ELISA檢測(cè)試劑盒
?有效期: 4 個(gè)月(4℃);8 個(gè)月(-20℃)。
?保存方法: 2-8℃(頻繁使用時(shí)); -20℃(長(zhǎng)時(shí)間不用時(shí))。
?品牌:子科生物ZIKER,美國R&D,美國immonoway,美國sciencell,德國IBL
?質(zhì)量保證:我告訴所有產(chǎn)品均需要經(jīng)過質(zhì)檢通過后,才允許出庫!
?適用范圍:實(shí)驗(yàn)結(jié)果僅供科研參考,不推薦用于臨床診斷結(jié)果。
人胰島素原(PI)ELISA檢測(cè)試劑盒子科生物現(xiàn)貨供應(yīng),子科生物進(jìn)口elisa試劑盒,國產(chǎn)elisa試劑盒,elisa試劑盒說明書,elisa試劑盒免費(fèi)代測(cè),ELISA試劑盒技術(shù)服務(wù),如需Elisa試劑盒說明書及報(bào)價(jià),可我司。
人胰島素原(PI)ELISA檢測(cè)試劑盒實(shí)驗(yàn)操作注意事項(xiàng)
1.試劑準(zhǔn)備:所有試劑都必須在使用前達(dá)到室溫,使用后請(qǐng)立即按照說明書要求保存。實(shí)驗(yàn)操作中必須使用一次性吸頭,避免交叉污染。
2.加樣:加樣時(shí),要控制加樣速度,避免*孔與zui后一孔之見的時(shí)間間隔過大,否則將會(huì)導(dǎo)致不同的預(yù)孵育時(shí)間,從而影響實(shí)驗(yàn)的準(zhǔn)確性以及重復(fù)性;一般加樣時(shí)間控制在10分鐘內(nèi),如果樣本數(shù)量過多,可使用多道移液器。
3.孵育:樣品要在密閉的容器內(nèi)進(jìn)行孵育,嚴(yán)格按照說明書上規(guī)定的孵育時(shí)間和溫度進(jìn)行。
4.洗滌:洗滌過程中反應(yīng)孔中的殘留的洗滌液應(yīng)在濾紙上充分拍干,同時(shí)要消除板底殘留的液體和手指痕跡,避免影響zui后的酶標(biāo)儀讀數(shù)。
5.反應(yīng)時(shí)間的控制:加入底物后請(qǐng)定時(shí)觀察反應(yīng)孔的顏色變化(比如,10分鐘左右),如果顏色較深,請(qǐng)?zhí)崆凹尤虢K止液終止反應(yīng)。
6.建議實(shí)驗(yàn)前預(yù)測(cè)樣品含量,如樣品濃度過高,應(yīng)對(duì)樣品進(jìn)行稀釋,計(jì)算結(jié)果時(shí)乘以相應(yīng)的稀釋倍數(shù)。
7.建議使用本試劑盒時(shí)先做預(yù)實(shí)驗(yàn)(即先做標(biāo)準(zhǔn)曲線,試用幾個(gè)標(biāo)本),如果對(duì)本試劑盒有任何疑問,可和所購經(jīng)銷商,如果因運(yùn)輸過程導(dǎo)致試劑盒失效,可要求調(diào)換,但概不承擔(dān)產(chǎn)品本身以外的任何損失。
標(biāo)本的采集及保存:
1.血清:標(biāo)本請(qǐng)于室溫放置2小時(shí)或4℃過夜后于1000 x g離心20分鐘,取上清即可檢測(cè),或?qū)?biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融。
2.血漿:可用EDTA或肝素作為抗凝劑,標(biāo)本采集后30分鐘內(nèi)于2 - 8° C 1000 x g離心15分鐘,或?qū)?biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融。
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小鼠谷胱甘肽S轉(zhuǎn)移酶θ1(GSTθ1) ELISA Kit 96T 48T 0.156-10 ng/mL 0.05 ng/mL mouse Glutathione S Transferase Theta 1 (GSTt1) ELISA Kit Mus musculus mouse
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人趨化因子C-C-基元配體14(CCL14) ELISA Kit 96T 48T 15.625-1000 pg/mL 6.8 pg/mL human Chemokine C-C-Motif Ligand 14 (CCL14) ELISA Kit Homo sapiens human
大鼠皮敵菌素(DCD) ELISA Kit 96T 48T 0.156-10ng/mL 0.057ng/mL Rat Dermcidin (DCD) ELISA Kit Rattus norvegicus Rat
人丙氨酸氨肽酶(AAP) ELISA Kit 96T 48T 1.563-100ng/mL 0.51ng/mL human Alanine Aminopeptidase (AAP) ELISA Kit Homo sapiens human
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大鼠Ⅰ型膠原α1(COL1α1) ELISA Kit 96T 48T 1.563-100ng/mL 0.61ng/mL Rat Collagen Type I Alpha 1 (COL1a1) ELISA Kit Rattus norvegicus Rat
人解整合素金屬蛋白酶17(ADAM17) ELISA Kit 96T 48T 0.156-10ng/mL 0.056ng/mL human A Disintegrin And Metalloprotease 17 (ADAM17) ELISA Kit Homo sapiens human
人S100鈣結(jié)合蛋白A7(S100A7) ELISA Kit 96T 48T 125-8000pg/mL 51pg/mL human S100 Calcium Binding Protein A7 (S100A7) ELISA Kit Homo sapiens human
牛免疫球蛋白結(jié)合蛋白1(IGBP1) ELISA Kit 96T 48T 0.156-10 ng/mL 0.041 ng/mL Bovine Immunoglobulin Binding Protein 1 (IGBP1) ELISA Kit Bos taurus; Bovine Cattle
山羊黃體激素(LH) ELISA Kit 96T 48T 1.23-100ng/mL 0.38ng/mL Goat Luteinizing Hormone (LH) ELISA Kit Capra hircus; Caprine Goat
小鼠微管關(guān)聯(lián)蛋白τ(MAPτ) ELISA Kit 96T 48T 7.813-500pg/mL 2.9pg/mL mouse Microtubule Associated Protein Tau (MAPt) ELISA Kit Mus musculus mouse
大鼠微管關(guān)聯(lián)蛋白τ(MAPτ) ELISA Kit 96T 48T 7.813-500pg/mL 2.5pg/mL Rat Microtubule Associated Protein Tau (MAPt) ELISA Kit Rattus norvegicus Rat
小鼠血幼素(HJV) ELISA Kit 96T 48T 0.625-40ng/mL 0.256ng/mL mouse Hemojuvelin (HJV) ELISA Kit Mus musculus mouse
人Ⅱ型膠原α1(COL2α1) ELISA Kit 96T 48T 0.625-40ng/mL 0.227ng/mL human Collagen Type II Alpha 1 (COL2a1) ELISA Kit Homo sapiens human
小鼠乙醇脫氫酶1(ADH1) ELISA Kit 96T 48T 0.313-20 ng/mL 0.09 ng/mL mouse Alcohol Dehydrogenase 1 (ADH1) ELISA Kit Mus musculus mouse
小鼠線粒體乙醛脫氫酶(ALDM) ELISA Kit 96T 48T 0.313-20 ng/mL 0.12 ng/mL mouse Aldehyde Dehydrogenase, Mitochondrial (ALDM) ELISA Kit Mus musculus mouse
小鼠乙醇脫氫酶7(ADH7) ELISA Kit 96T 48T 0.313-20 ng/mL 0.13 ng/mL mouse Alcohol Dehydrogenase 7 (ADH7) ELISA Kit Mus musculus mouse
人磷酸甘油酸酯激酶1(PGK1) ELISA Kit 96T 48T 0.313-20ng/mL 0.123ng/mL human Phosphoglycerate Kinase 1 (PGK1) ELISA Kit Homo sapiens human
人過氧化氫酶(CAT) ELISA Kit 96T 48T 0.313-20ng/mL 0.133ng/mL human Catalase (CAT) ELISA Kit Homo sapiens human
豬肝型脂肪酸結(jié)合蛋白(FABP1) ELISA Kit 96T 48T 0.156-10ng/mL 0.060ng/mL porcine Fatty Acid Binding Protein 1, Liver (FABP1) ELISA Kit Sus scrofa; Porcine Pig
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豬血管內(nèi)皮生長(zhǎng)因子受體1(VEGFR1) ELISA Kit 96T 48T 0.313-20ng/mL 0.115ng/mL porcine Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) ELISA Kit Sus scrofa; Porcine Pig
小鼠晚期糖基化終末產(chǎn)物受體(AGER) ELISA Kit 96T 48T 0.156-10ng/mL 0.058ng/mL mouse Advanced Glycosylation End Product Specific Receptor (AGER) ELISA Kit Mus musculus mouse
人載脂蛋白M(APOM) ELISA Kit 96T 48T 0.313-20ng/mL 0.113ng/mL human Apolipoprotein M (APOM) ELISA Kit Homo sapiens human
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人脂肪酸合酶(FASN) ELISA Kit 96T 48T 0.313-20ng/mL 0.124ng/mL human Fatty Acid Synthase (FASN) ELISA Kit Homo sapiens human
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人乳脂球表皮生長(zhǎng)因子8(MFGE8) ELISA Kit 96T 48T 31.25-2000pg/mL 12.3pg/mL human Milk Fat Globule EGF Factor 8 (MFGE8) ELISA Kit Homo sapiens human
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