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人糖化白蛋白(GA)ELISA檢測試劑盒進口/國產(chǎn)
?產(chǎn)品規(guī)格:96T(人份)/48T(人份)(單孔檢測分別可以檢測90份樣本,42分樣本,一般都需要留6個孔做標準曲線)。
?標本:血清、血漿、細胞上清液、尿液、體液、灌洗液、腦脊髓、心房水、胸房水、組織等
?運輸:全國各地快遞免費送貨上門,低溫運輸,用冰袋加泡沫保溫盒包裝,確保產(chǎn)品運輸狀態(tài)下也處于低溫狀態(tài)。
人糖化白蛋白(GA)ELISA檢測試劑盒進口/國產(chǎn)包含內(nèi)容:操作過程中的問題注意事項
1)加樣
對于間接ELISA標本一般都要進行稀釋,如果加樣不準就會造成誤差,尤其當稀釋倍數(shù)大時,很小的誤差,會導致較大的相對誤差,使陰性(或弱陽性)標本呈陽性(或陰性)。加樣時應將所加物加在ELISA板孔的底部,避免加在孔壁上部,并注意不可濺出,不可產(chǎn)生氣泡。目前,大部分血站都已使用全自動酶免加樣系統(tǒng)處理標本,可較好地避免以上誤差。
2)洗滌
在ELISA中正確的洗滌是保證得到可重復結果的關健一步,應引起操作者重視。無論是手工操作還是機器操作,得出不正確的結果常與不正確的洗滌有關,ELISA就是靠洗滌來達到分離游離和結合的酶標記物的目的。通過洗滌以消除殘留在板孔中沒能與固體抗原或抗體結合的物質,以及在反應過程中非特異性吸附于固相載體的干擾物質。
3) 溫育
每種試劑都有其佳反應模式,其中溫度和溫育時間控制是重要因素。因孵育溫度高,反應時間長,會造成整板本底高,陽性率高。溫育一般用濕盒或水浴,反應板不宜疊放,以保證各板溫度都能迅速平衡,為避免蒸發(fā),板上應加蓋。
4)酶標儀判讀
作為記錄測定結果的儀器,酶標儀的性能穩(wěn)定與否,決定結果的可靠度。首先酶標儀應定期進行保養(yǎng),對濾光片要定期校正;其次酶標儀波長設置要正確,使用雙波長,一個檢測波長,一個參比波長,以消除微孔板底部劃痕、不平、指印或液面高度差異造成的光干擾。此外,在用酶標儀讀數(shù)時先擦試微孔板底部并壓平板條。由于各種酶標儀性能有所不同。
牛泛素(Ub) ELISA Kit 96T 48T 0.156-10ng/mL 0.059ng/mL Bovine Ubiquitin (Ub) ELISA Kit Bos taurus; Bovine Cattle
犬泛素(Ub) ELISA Kit 96T 48T 6.25-400 ng/mL 1.6 ng/mL Canine Ubiquitin (Ub) ELISA Kit Canis familiaris; Canine Dog
馬泛素(Ub) ELISA Kit 96T 48T 12.5-800 ng/mL 2.8 ng/mL Equine Ubiquitin (Ub) ELISA Kit Equus caballus; Equine Horse
雞泛素(Ub) ELISA Kit 96T 48T 3.125-200 ng/mL 0.74 ng/mL Chicken Ubiquitin (Ub) ELISA Kit Chicken Gallus
豚鼠泛素(Ub) ELISA Kit 96T 48T 1.563-100 ng/mL 0.36 ng/mL Guinea pig Ubiquitin (Ub) ELISA Kit Cavia Guinea pig
豬泛素(Ub) ELISA Kit 96T 48T 9.375-600 ng/mL 2.34 ng/mL porcine Ubiquitin (Ub) ELISA Kit Sus scrofa; Porcine Pig
兔泛素(Ub) ELISA Kit 96T 48T 3.125-200 ng/mL 0.78 ng/mL Rabbit Ubiquitin (Ub) ELISA Kit Oryctolagus cuniculus Rabbit
猴泛素(Ub) ELISA Kit 96T 48T 6.25-400 ng/mL 2.1 ng/mL Monkey Ubiquitin (Ub) ELISA Kit Rhesus monkey Simian
牛血管生長素(ANG) ELISA Kit 96T 48T 15.625-1000pg/mL 6.1pg/mL Bovine Angiogenin (ANG) ELISA Kit Bos taurus; Bovine Cattle
犬白介素1α(IL1α) ELISA Kit 96T 48T 15.625-1000pg/mL 6.6pg/mL Canine Interleukin 1 Alpha (IL1a) ELISA Kit Canis familiaris; Canine Dog
山羊白介素1α(IL1α) ELISA Kit 96T 48T 15.625-1000 pg/mL 7.1 pg/mL Goat Interleukin 1 Alpha (IL1a) ELISA Kit Capra hircus; Caprine Goat
馬白介素1α(IL1α) ELISA Kit 96T 48T 7.813-500pg/mL 2.7pg/mL Equine Interleukin 1 Alpha (IL1a) ELISA Kit Equus caballus; Equine Horse
豚鼠白介素1α(IL1α) ELISA Kit 96T 48T 15.625-1000pg/mL 6.6pg/mL Guinea pig Interleukin 1 Alpha (IL1a) ELISA Kit Cavia Guinea pig
兔白介素1α(IL1α) ELISA Kit 96T 48T 15.625-1000pg/mL 5.4pg/mL Rabbit Interleukin 1 Alpha (IL1a) ELISA Kit Oryctolagus cuniculus Rabbit
山羊防御素β2(DEFβ2) ELISA Kit 96T 48T 0.156-10 ng/mL 0.062 ng/mL Goat Defensin Beta 2 (DEFb2) ELISA Kit Capra hircus; Caprine Goat
馬防御素β2(DEFβ2) ELISA Kit 96T 48T 78.125-5000pg/mL 28pg/mL Equine Defensin Beta 2 (DEFb2) ELISA Kit Equus caballus; Equine Horse
小鼠防御素β2(DEFβ2) ELISA Kit 96T 48T 3.125-200pg/mL 1.31pg/mL mouse Defensin Beta 2 (DEFb2) ELISA Kit Mus musculus mouse
馬白介素2(IL2) ELISA Kit 96T 48T 31.25-2000pg/mL 10.5pg/mL Equine Interleukin 2 (IL2) ELISA Kit Equus caballus; Equine Horse
小鼠免疫球蛋白G1(IgG1) ELISA Kit 96T 48T 617.28-50000ng/mL 264.5ng/mL mouse Immunoglobulin G1 (IgG1) ELISA Kit Mus musculus mouse
大鼠基質金屬蛋白酶12(MMP12) ELISA Kit 96T 48T 78.125-5000pg/mL 32pg/mL Rat Matrix Metalloproteinase 12 (MMP12) ELISA Kit Rattus norvegicus Rat
牛半乳糖苷酶β(GLβ) ELISA Kit 96T 48T 9.375-600 U/L 4.05 U/L Bovine Galactosidase Beta (GLb) ELISA Kit Bos taurus; Bovine Cattle
犬半乳糖苷酶β(GLβ) ELISA Kit 96T 48T 0.313-20 ng/mL 0.13 ng/mL Canine Galactosidase Beta (GLb) ELISA Kit Canis familiaris; Canine Dog
魚半乳糖苷酶β(GLβ) ELISA Kit 96T 48T 0.781-50 U/L 0.195 U/L fish Galactosidase Beta (GLb) ELISA Kit Danio rerio Zebrafish
猴半乳糖苷酶β(GLβ) ELISA Kit 96T 48T 3.125-200 U/L 1.54 U/L Monkey Galactosidase Beta (GLb) ELISA Kit Rhesus monkey Simian
牛前動力蛋白受體1(PKR1) ELISA Kit 96T 48T 0.938-60 ng/mL 0.23 ng/mL Bovine Prokineticin Receptor 1 (PKR1) ELISA Kit Bos taurus; Bovine Cattle
大鼠前動力蛋白受體1(PKR1) ELISA Kit 96T 48T 0.156-10 ng/mL 0.039 ng/mL Rat Prokineticin Receptor 1 (PKR1) ELISA Kit Rattus norvegicus Rat
牛親環(huán)素B(CYPB) ELISA Kit 96T 48T 12.5-800 ng/mL 5.2 ng/mL Bovine Cyclophilin B (CYPB) ELISA Kit Bos taurus; Bovine Cattle
雞親環(huán)素B(CYPB) ELISA Kit 96T 48T 3.125-200 ng/mL 1.3 ng/mL Chicken Cyclophilin B (CYPB) ELISA Kit Chicken Gallus