上海研盟生物有限公司*品質(zhì)供應(yīng)Anti-PAICS抗體,我公司銷售國內(nèi)外科研產(chǎn)品,如生物試劑、生化試劑,一抗二抗,各種標(biāo)記抗體,Elisa酶聯(lián)免疫試劑盒,放免試劑盒,培養(yǎng)基,各種動(dòng)物血清等產(chǎn)品,歡迎廣大新老客戶前來咨詢訂購,訂購:或。
磷酸核糖胺咪唑羧化酶抗體,Anti-PAICS抗體簡單介紹:
中文名稱:磷酸核糖胺咪唑羧化酶抗體
英文名稱:Anti-PAICS抗體
別 名:ADE2; ADE2H1; AIR carboxylase; AIRC; DKFZp781N1372; MGC1343; MGC5024; Paics; PAIS; Phosphoribosylaminoimidazole carboxylase; PUR6_HUMAN; SAICAR synthetase.
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
背景介紹:This gene encodes a bifunctional enzyme containing phosphoribosylaminoimidazole carboxylase activity in its N-terminal region and phosphoribosylaminoimidazole succinocarboxamide synthetase in its C-terminal region. It catalyzes steps 6 and 7 of purine biosynthesis. The gene is closely linked and divergently transcribed with a locus that encodes an enzyme in the same pathway, and transcription of the two genes is coordinay regulated. The human genome contains several pseudogenes of this gene. Multiple transcript variants encoding different isoforms have been found for this gene.
規(guī)格:0.1ml/0.2ml
濃度:1mg/1ml
抗體來源:本公司抗體有兔來源和鼠來源等
克隆類型:兔來源為多抗,鼠來源為單抗
產(chǎn)品類型:一抗
磷酸核糖胺咪唑羧化酶抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
避免反復(fù)凍融,保質(zhì)期一年。
磷酸核糖胺咪唑羧化酶抗體,Anti-PAICS抗體免疫組化問題解答
1、染色過強(qiáng)?
原 因:抗體濃度過高或孵育時(shí)間過長
解決方法:降低抗體滴度、抗體孵育時(shí)間:室溫1小時(shí)或4度過夜
原 因:孵育溫度過高超過37度
解決方法:一般在室溫20-28度
原 因:DAB顯色時(shí)間過長或濃度過高
解決方法:顯色時(shí)間不超過5-12分鐘,以顯微鏡下觀察為準(zhǔn)
2、非特異性背景染色?
原 因:操作過程中沖洗不充分
解決方法:每步?jīng)_洗3次每次5分鐘
原 因:組織中含過氧化物酶未阻斷
解決方法:可再配置新鮮3%H2O2封閉孵育時(shí)間延長
原 因:組織中含內(nèi)原性*
解決方法:正常非免疫動(dòng)物血清再封閉
原 因:血清蛋白封閉不充分
解決方法:血清蛋白封閉不充分
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