蘑菇假單胞菌說明書低溫存法:
①簡單保存法,將瓊脂斜面孢子培養(yǎng)物、菌絲懸浮液以及由麩皮、大米、小米等谷物原料制成的孢子培養(yǎng)物置于4℃冰箱保存,保存時間不超過1~2個月,若將谷物原料制備的孢子瓶抽真空并在棉塞上浸蠟,以隔絕外界空氣和水汽,保持時間可達(dá)3~4個月;②液氮超低溫保存法,將生長穩(wěn)定期的細(xì)胞懸浮在10%甘油或其他低冰點(diǎn)液體中,密封于安瓿管內(nèi),然后控制冷卻速度,使安瓿管溫度逐步下降至 -35℃時,即可置于-150~-196℃的液氮罐中保存。大多數(shù)微生物如病毒、噬菌體、多種細(xì)菌、放線菌、酵母和原蟲、特別是一些用冷凍干燥法有困難的微生物,都可用此法*保存。
產(chǎn)品名稱: 蘑菇假單胞菌說明書
英文名稱:Mushroom Pseudomonas
型號:CS-JZ0002
培養(yǎng)保藏法:培養(yǎng)后于4—6℃冰箱內(nèi)保存。
庫存:提供現(xiàn)貨
保存:低溫避光保存
注意事項(xiàng):
l、微生物菌種應(yīng)保藏于低溫、清潔和干燥的地方,室溫放置時問過長會導(dǎo)致菌種衰退;
2、菌種操作應(yīng)在無菌條件下進(jìn)行,防止雜菌污染:
3、斜面菌種保藏時間通常為1-2個月,應(yīng)根據(jù)菌種狀況及時轉(zhuǎn)接;凍干菌種保藏時間通常為5~10年;
4、菌種使用過程中如出現(xiàn)雜菌污染或菌種生產(chǎn)性能下降,應(yīng)及時與我公司或更換新的菌種。
作用機(jī)理及使用效果
1、耗氧產(chǎn)酸,調(diào)節(jié)腸道微生態(tài)平衡,達(dá)到預(yù)防腹瀉、下痢等疾病
枯草芽孢桿菌在生長過程中耗氧,造成腸道低氧,并產(chǎn)生丙酸、丁酸等有機(jī)酸降低腸道內(nèi)PH值,促進(jìn)有益厭氧菌如乳酸菌、雙歧桿菌等的生長,同時抑制腸道病原細(xì)菌如大腸桿菌、沙門氏菌等的生長繁殖,提高有益微生物在腸道細(xì)菌種間的競爭優(yōu)勢,達(dá)到預(yù)防豬腹瀉及下痢的目的。
2、分泌多種酶類,提高飼料消化利用率
枯草芽孢桿菌可分泌多種酶如蛋白酶、*、脂肪酶、果膠酶、葡聚糖酶等,降解動植物性飼料中復(fù)雜有機(jī)物,促進(jìn)消化吸收,提高飼料利用率。
3、產(chǎn)生多種營養(yǎng)素,參與機(jī)體新陳代謝
枯草芽孢桿菌在動物腸道內(nèi)生長繁殖,能產(chǎn)生維生素、促生長因子和蛋白多肽類抗菌物質(zhì)等多種營養(yǎng)物質(zhì),還可以提高動物對鈣、磷的利用率,參與機(jī)體的新陳代謝。減少糞便中氮、磷、鈣的排泄量,減少有害氣體排放,降低養(yǎng)殖場所氨氣、吲哚等有害氣體的濃度,減輕糞便臭味,改善養(yǎng)殖環(huán)境。
4、提高動物免疫力
枯草芽孢桿菌能夠刺激動物免疫器官的生長發(fā)育,激活淋巴細(xì)胞,提高免疫球蛋白和抗體水平,通過淋巴循環(huán)活化全身的免疫防御系統(tǒng),提高動物免疫力和抗病力。同時緩解厭食,生長緩慢等應(yīng)激反應(yīng)。
英文名稱 Anti-GNB1L
中文名稱 G蛋白β樣蛋白抗體
別 名 DGCRK3; ESTM55; fb98e06; fj09d12; FKSG1; G-protein beta subunit-like protein; guanine nucleotide binding protein (G protein), beta polypeptide 1-like; guanine nucleotide binding protein beta-subunit-like polypeptide; GY2; KIAA1645; WDR14; WDVCF; wu:fb98e06; wu:fj09d12; zgc:55774.
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Dog, Horse, Zebrafish
產(chǎn)品類型 一抗
研究領(lǐng)域 心血管 細(xì)胞生物 信號轉(zhuǎn)導(dǎo) G蛋白偶聯(lián)受體 G蛋白信號
蛋白分子量 predicted molecular weight: 36kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human GNB1L
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
(石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 This gene encodes a G-protein beta-subunit-like polypeptide which is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximay 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains 6 WD repeats and is highly expressed in the heart. The gene maps to the region on chromosome 22q11, which is deleted in DiGeorge syndrome, trisomic in derivative 22 syndrome and tetrasomic in cat-eye syndrome. Therefore, this gene may contribute to the etiology of those disorders. Transcripts from this gene share exons with some transcripts from the C22orf29 gene. [provided by RefSeq, Jul 2008].
Function : GNB1L is a G-protein beta-subunit-like polypeptide which is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximay 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains 6 WD repeats and is highly expressed in the heart. Therefore, this gene may contribute to the etiology of those disorders.This gene encodes a G-protein beta-subunit-like polypeptide which is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximay 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains 6 WD repeats and is highly expressed in the heart. The gene maps to the region on chromosome 22q11, which is deleted in DiGeorge syndrome, trisomic in derivative 22 syndrome and tetrasomic in cat-eye syndrome. Therefore, this gene may contribute to the etiology of those disorders.
Tissue Specificity : Ubiquitous. Highly expressed in heart, liver, skeletal muscle, kidney, spleen, thymus and pancreas. Detected at low levels in lung, placenta and brain.
Similarity : Contains 6 WD repeats.
Database links : UniProtKB/Swiss-Prot: Q9BYB4.2
英文名稱 Anti-GNB5
中文名稱 G蛋白β5/Gβ 5 抗體
別 名 FLJ37457; FLJ43714; G protein beta 5 subunit; G protein beta subunit 5L; GB 5; GB5; GBB5_HUMAN; Gbeta5; GNB 5; GNB5; Guanine nucleotide binding protein (G protein) beta 5; Guanine nucleotide binding protein beta 5; Guanine nucleotide binding protein beta 5 subunit; Guanine nucleotide binding protein beta subunit 5L; Guanine nucleotide binding protein subunit beta 5; Guanine nucleotide-binding protein subunit beta-5; Transducin beta chain 5.
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit, Sheep
產(chǎn)品類型 一抗
研究領(lǐng)域 細(xì)胞生物 信號轉(zhuǎn)導(dǎo) G蛋白偶聯(lián)受體 G蛋白信號
蛋白分子量 predicted molecular weight: 44kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human GNB5
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
(石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Alternatively spliced transcript variants encoding different isoforms exist. [provided by RefSeq, Jul 2008].
Function : Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
Subunit : G proteins are composed of 3 units, alpha, beta and gamma. Component of the RGS9-1-Gbeta5 complex composed of RGS9 (isoform RGS9-1), Gbeta5 (GNB5) and RGS9BP (By similarity).
Tissue Specificity : Expressed in multiple tissues.
Similarity : Belongs to the WD repeat G protein beta family.
Contains 7 WD repeats.
Database links : UniProtKB/Swiss-Prot: O14775.2