產(chǎn)堿假單胞菌培養(yǎng)低溫存法:
①簡單保存法,將瓊脂斜面孢子培養(yǎng)物、菌絲懸浮液以及由麩皮、大米、小米等谷物原料制成的孢子培養(yǎng)物置于4℃冰箱保存,保存時間不超過1~2個月,若將谷物原料制備的孢子瓶抽真空并在棉塞上浸蠟,以隔絕外界空氣和水汽,保持時間可達(dá)3~4個月;②液氮超低溫保存法,將生長穩(wěn)定期的細(xì)胞懸浮在10%甘油或其他低冰點液體中,密封于安瓿管內(nèi),然后控制冷卻速度,使安瓿管溫度逐步下降至 -35℃時,即可置于-150~-196℃的液氮罐中保存。大多數(shù)微生物如病毒、噬菌體、多種細(xì)菌、放線菌、酵母和原蟲、特別是一些用冷凍干燥法有困難的微生物,都可用此法*保存。
產(chǎn)品名稱: 產(chǎn)堿假單胞菌培養(yǎng)
英文名稱:Pseudomonas Alcaligenes
型號:CS-JZ0003培養(yǎng)保藏法:培養(yǎng)后于4—6℃冰箱內(nèi)保存。
庫存:提供現(xiàn)貨
保存:低溫避光保存
注意事項:
l、微生物菌種應(yīng)保藏于低溫、清潔和干燥的地方,室溫放置時問過長會導(dǎo)致菌種衰退;
2、菌種操作應(yīng)在無菌條件下進行,防止雜菌污染:
3、斜面菌種保藏時間通常為1-2個月,應(yīng)根據(jù)菌種狀況及時轉(zhuǎn)接;凍干菌種保藏時間通常為5~10年;
4、菌種使用過程中如出現(xiàn)雜菌污染或菌種生產(chǎn)性能下降,應(yīng)及時與我公司或更換新的菌種。
作用機理及使用效果
1、耗氧產(chǎn)酸,調(diào)節(jié)腸道微生態(tài)平衡,達(dá)到預(yù)防腹瀉、下痢等疾病
枯草芽孢桿菌在生長過程中耗氧,造成腸道低氧,并產(chǎn)生丙酸、丁酸等有機酸降低腸道內(nèi)PH值,促進有益厭氧菌如乳酸菌、雙歧桿菌等的生長,同時抑制腸道病原細(xì)菌如大腸桿菌、沙門氏菌等的生長繁殖,提高有益微生物在腸道細(xì)菌種間的競爭優(yōu)勢,達(dá)到預(yù)防豬腹瀉及下痢的目的。
2、分泌多種酶類,提高飼料消化利用率
枯草芽孢桿菌可分泌多種酶如蛋白酶、*、脂肪酶、果膠酶、葡聚糖酶等,降解動植物性飼料中復(fù)雜有機物,促進消化吸收,提高飼料利用率。
3、產(chǎn)生多種營養(yǎng)素,參與機體新陳代謝
枯草芽孢桿菌在動物腸道內(nèi)生長繁殖,能產(chǎn)生維生素、促生長因子和蛋白多肽類抗菌物質(zhì)等多種營養(yǎng)物質(zhì),還可以提高動物對鈣、磷的利用率,參與機體的新陳代謝。減少糞便中氮、磷、鈣的排泄量,減少有害氣體排放,降低養(yǎng)殖場所氨氣、吲哚等有害氣體的濃度,減輕糞便臭味,改善養(yǎng)殖環(huán)境。
4、提高動物免疫力
枯草芽孢桿菌能夠刺激動物免疫器官的生長發(fā)育,激活淋巴細(xì)胞,提高免疫球蛋白和抗體水平,通過淋巴循環(huán)活化全身的免疫防御系統(tǒng),提高動物免疫力和抗病力。同時緩解厭食,生長緩慢等應(yīng)激反應(yīng)。
英文名稱 Anti-GNPNAT1/GNA1
中文名稱 葡萄糖6-N-乙?;D(zhuǎn)移酶1抗體
別 名 AU017428; AU040593; EMeg32; FLJ10607; Glucosamine 6 phosphate acetyltransferase; Glucosamine 6 phosphate N acetyltransferase; Glucosamine 6-phosphate N-acetyltransferase; Glucosamine phosphate N acetyltransferase 1; GNA1; GNA1_HUMAN; GNPNAT; GNPNAT1; Gpnat1; Gsnpat; Phosphoglucosamine acetylase; Phosphoglucosamine transacetylase; RGD1563144.
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Pig, Cow, Rabbit
產(chǎn)品類型 一抗
研究領(lǐng)域
蛋白分子量 predicted molecular weight: 21kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human GNPNAT1/GNA1
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
(石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 Glucosamine 6-phosphate N-acetyltransferase (GNA1), also designated phosphoglucosamine transacetylase or phosphoglucosamine acetylase, belongs to the GNA1 subfamily of the larger acetyltransferase family of proteins. GNA1, a peripheral membrane protein containing one N-acetyltransferase domain, is expressed in the colon and maps to cytoband 14q22.1. The protein localizes to the Golgi apparatus and the endosome. It is important for UDP-GlcNAc biosynthesis pathway. GNA1 catalyzes the synthesis of GlcNAc6P from AcCoA and GlcN6P, a step in the UDP-GlcNAc6P formation pathway.
Function : Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I): step 1/2.
Subcellular Location : Golgi apparatus membrane. Endosome membrane.
Similarity : Belongs to the acetyltransferase family.
GNA1 subfamily.
Contains 1 N-acetyltransferase domain.
Database links : UniProtKB/Swiss-Prot: Q96EK6.1
英文名稱 Anti-GNA14
中文名稱 G蛋白偶聯(lián)受體結(jié)合蛋白α14/Gα 14 抗體
別 名 AU023208; g alpha 14; G alpha-14; G protein alpha subunit 14; G protein subunit alpha 14; G-protein subunit alpha-14; gna14; GNA14_HUMAN; Guanine nucleotide binding protein (G protein) alpha 14; Guanine nucleotide binding protein 14; Guanine nucleotide binding protein alpha 14 subunit; Guanine nucleotide binding protein subunit alpha 14; Guanine nucleotide-binding protein subunit alpha-14; MGC105546; OTTHUMP00000021515; Tieg3.
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep
產(chǎn)品類型 一抗
研究領(lǐng)域 細(xì)胞生物 信號轉(zhuǎn)導(dǎo) G蛋白偶聯(lián)受體 G蛋白信號
蛋白分子量 predicted molecular weight: 42kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human GNA14
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
(石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 This gene encodes a member of the guanine nucleotide-binding, or G protein family. G proteins are heterotrimers consisting of alpha, beta and gamma subunits. The encoded protein is a member of the alpha family of G proteins, more specifically the alpha q subfamily of G proteins. The encoded protein may play a role in pertussis-toxin resistant activation of phospholipase C-beta and its downstream effectors.[provided by RefSeq, Feb 2009].
Function : Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.
Subunit : G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.
Similarity : Belongs to the G-alpha family. G(q) subfamily.
Database links : UniProtKB/Swiss-Prot: O95837.1