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聯(lián)系我時，請告知來自 環(huán)保在線Arf1ProteinΔ17Q71Lmutant[10123][10µg]CatalogNumber:10123ProductName:Arf1ProteinΔ17Q71LmutantSynonyms:ADP-ribosylationfactor1Source:Humanrecombinant,His6-tagExpressionHost:E
Arf1 Protein Δ17Q71L mutant [10123]
[10 µg]
| Catalog Number: 10123 |
| Product Name: Arf1 Protein Δ17Q71L mutant |
| Synonyms: ADP-ribosylation factor 1 |
| Source: Human recombinant, His6-tag |
| Expression Host: E. coli |
| Molecular Weight: 21 kDa |
| Purity: >95% by SDS-PAGE |
| Introduction: Arf1 is a member of the ARF super-family. ARF genes encode small GTPases that increase the ADP-ribosyltransferase activity of cholera toxin and are critical for vesicular trafficking as activators of phospholipase D. Arf1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. |
| Amino Acid Sequence (1-181, Δ17, Q71L) |
| MGNIFANLFKGLFGKK-MRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWD VGGLDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAM NAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQK |
| Properties |
| Physical Appearance (form): Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl. |
| Physical Appearance (form): White or clear |
| Concentration: 1 mg/mL |
| Storage: -80°C |
| Preparation Instructions: Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside(DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Arf1 Δ17Q71L was determined by SDS-PAGE and Coomassie Brilliant Blue Staining |
| References: 1. Amor, J. C. et al., Nature 372: 704-708, 1994. 2. Bobak, D. A. et al., Proc. Nat. Acad. Sci. 86: 6101-6105, 1989. 3. Hirai, M. et al., Genomics 34: 263-265, 1996. 4. Kumari, S. et al., Cell Biol. 10: 30-41, 2008. 5. Lee, C.-M. et al., J. Biol. Chem. 267: 9028-9034, 1992. 6. Mossessova, E. et al., Cell 92: 415-423, 1998. 7. Peng, Z. G. et al., Biofactors 2: 45-49, 1989. 8. Presley, J. F. et al., Nature 417: 187-193, 2002. 9. Renault, L. et al., Nature 426: 525-530, 2003. |
| Datasheet: |
| Related Products | |||
| Catalog# | Name | Size | Price |
| Arf1 Protein Δ17Q71L mutant | 100 μg | ￥2,800 | |
| Arf1 Protein Δ17 mutant | 100 μg | ￥2,800 | |
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