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Anti FGFR1(V664L) Mouse Monoclonal Antibody

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FGFR1(V664L)[26259][100ul]CatalogNumber:26259GeneSymbol:FGFR1

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FGFR1(V664L) [26259]
[100ul]

Catalog Number: 26259

Gene Symbol: FGFR1,BFGFR,CD331,CEK,FGFBR,FLG

Description: Anti FGFR1(V664L) Mouse Monoclonal Antibody

Background: The protein FGFR1 is highly conserved between members and throughout evolution. This particular family member of FGFR1 binds both acidic and basic fibroblast growth factors and is involved in limb induction. Mutations of FGFR1 protein have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, squamous cell lung cancer and autosomal dominant Kallmann syndrome.

Immunogen: A synthetic peptide from the internal region of FGFR1 which includes the mutation of V664L, human origin.

Applications: ELISA, WB, IF, IHC

Recommended Dilutions:
ELISA: 1:1000-1:2000
WB: 1:500-1:1000
IF: 1:50-1:100
IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes V664L mutant, but not wild-type FGFR1 of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Download Datasheet:

Western blot:

Western blot analysis of recombinant FGFR1(V664L) and wildtype proteins. Purified His-tagged FGFR1(V664L) protein (478-767aa, 38KD, lane 2,) and corresponding wildtype protein (lane 1) were blotted with Anti FGFR1(V664L) monoclonal antibody (Cat. #26259).

Immunofluorescence:

Immunofluorescence of cells expressing FGFR1 proteins with Anti FGFR1(V664L) antibody. HEK293T cells were transfected with pCDNA3-GFP FGFR1(WT) plasmid (left column) or pCDNA3-GFP FGFR1(V664L) plasmid (right column), then fixed and stained with Anti FGFR1(V664L) monoclonal antibody (Cat. #26259).

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