EML4-ALK[26242][100ul]CatalogNumber:26242GeneSymbol:EML4-ALKDescription:AntiEML4ALKMouseMonoclonalAntibodyBackground:EML4-ALKisafusion-typeproteinkinasethatcanbefoundinmanytumors

詳細介紹

EML4-ALK [26242]
[100ul]

Catalog Number: 26242

Gene Symbol: EML4-ALK

Description: Anti EML4 ALK Mouse Monoclonal Antibody

Background: EML4-ALK is a fusion-type protein kinase that can be found in many tumors, such as anaplastic large cell lymphoma, inflammatory myofibroblastic tumor, neuroblastoma and NSCLC, and so on. It is generated as a result of a small inversion within the short arm of human chromosome 2. EML4-ALK protein often promotes and maintains the malignant behavior of the cancer cells by activating the MAPK, PI3K/AKT and JAK/STAT3 pathways.

Immunogen: A synthetic peptide from the internal region of EML4-ALK of human origin.

Applications: ELISA, WB, IHC

Recommended Dilutions:
ELISA: 1:1000-1:2000
WB: 1:500-1:1000
IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ ａnd Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes T41A mutant, but not wild-type EML4-ALK of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Download Datasheet:

Western blot:

Western blot analysis of recombinant EML4 ALK and wildtype proteins. Purified His-tagged EML4-ALK protein (lane 3), corresponding wild-type EML4 protein (lane1) and wild-type ALK protein (lane2) were blotted with Anti EML4 ALK monoclonal antibody (Cat. #26242).

Immunofluorescence:

Immunofluorescence of cells expressing EML4-ALK proteins with Anti EML4 ALK antibody. HEK293T cells were transfected with pCDNA3-GFP-EML4 (WT) plasmid (left column) or pCDNA3-GFP ALK(WT) plasmid (middle column) or pCDNA3-GFP-EML4-ALK plasmid (right column), then fixed and stained with Anti EML4 ALK monoclonal antibody (Cat. #26242).

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