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Anti ERBB2(D769H) Mouse Monoclonal Antibody

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ERBB2(D769H)[26381][100ul]CatalogNumber:26381GeneSymbol:ERBB2/HER2/Neu/c-NeuDescription:AntiERBB2(D769H)MouseMonoclonalAntibodyBackground:TheHER-2/neuoncogene

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ERBB2(D769H) [26381]
[100ul]

Catalog Number:  26381
Gene Symbol:  ERBB2/HER2/Neu/c-Neu
Description:   Anti ERBB2(D769H) Mouse Monoclonal Antibody
Background:   The HER-2/neu oncogene, a member of the epidermal growth factor receptor or erb-B gene-like family, encodes a transmembrane tyrosine kinase receptor that mediates extracellular signals activated by epidermal growth factors. Her2 abnormal has been strongly associated with many malignant tumors, especially with breast cancers. The expression level of Her2 is an important critiria in clinic evaluating of the progression of breast cancer.
Immunogen:   A synthetic peptide from the internal region of ERBB2 which includes the mutation of D769H, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:2000 
WB: 1:500-1:1000 
IF: 1:25-1:50
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes D769H mutant, but not wild-type ERBB2 of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
Download Datasheet:  
Western blot:  
Western blot analysis of recombinant ERBB2(D769H) and wildtype proteins. Purified His-tagged ERBB2(D769H) protein (lane 1) and corresponding wildtype protein (lane 2) were blotted with Anti ERBB2(D769H) monoclonal antibody (Cat. #26381).
Immunofluorescence:  
Immunofluorescence of cells expressing ERBB2 proteins with Anti ERBB2(D769H) antibody. HEK293T cells were transfected with pCDNA3-GFP ERBB2(WT) plasmid (left column) or pCDNA3-GFP ERBB2(D769H) plasmid (right column), then fixed and stained with Anti ERBB2(D769H) monoclonal antibody (Cat. #26381).
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Anti ERBB2(D769Y) Mouse Monoclonal Antibody 100 μL¥4800
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