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Anti KIT(D820E) Mouse Monoclonal Antibody

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參   考   價(jià): ￥ 4800

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KIT(D820E)[26274][100ul]CatalogNumber:26274GeneSymbol:C-Kit

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KIT(D820E) [26274]
[100ul]


Catalog Number:  26274
Gene Symbol:  C-Kit; CD117; KIT; PBT; SCFR
Description:   Anti KIT(D820E) Mouse Monoclonal Antibody
Background:   Tyrosine-protein kinase Kit, also known as proto-oncogene c-Kit or CD117 or Mast/stem cell growth factor receptor (SCFR), is a receptor tyrosine kinase protein that in humans is encoded by the KIT gene. Activating mutations in this gene are associated with gastrointestinal stromal tumors, testicular seminoma, mast cell disease, melanoma, acute myeloid leukemia, while inactivating mutations are associated with the genetic defect piebaldism.
Immunogen:   A synthetic peptide from the internal region of KIT which includes the mutation of D820E, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:500-1:1000 
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes D820E mutant, but not wild-type KIT of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
Download Datasheet:  
Western blot:  
Western blot analysis of recombinant KIT(D820E) and wild-type proteins. Purified His-tagged KIT(D820E) protein (lane 1) and corresponding wild-type protein (lane 2) were blotted with Anti KIT(D820E) monoclonal antibody (Cat. #26274).
Immunofluorescence:  
Immunofluorescence of cells expressing KIT proteins with Anti KIT(D820E) antibody. HEK293T cells were transfected with pCDNA3-GFP KIT(WT) plasmid (left column) or pCDNA3-GFP KIT(D820E) plasmid (right column), then fixed and stained with anti KIT(D820E) monoclonal antibody (Cat. #26274).


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