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Anti KIT(V560DEL) Mouse Monoclonal Antibody

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KIT(V560DEL)[26289][100ul]CatalogNumber:26289GeneSymbol:C-Kit

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KIT(V560DEL) [26289]
[100ul]

Catalog Number: 26289

Gene Symbol: C-Kit; CD117; KIT; PBT; SCFR

Description: Anti KIT(V560DEL) Mouse Monoclonal Antibody

Background: Tyrosine-protein kinase Kit, also known as proto-oncogene c-Kit or CD117 or Mast/stem cell growth factor receptor (SCFR), is a receptor tyrosine kinase protein that in humans is encoded by the KIT gene. Activating mutations in this gene are associated with gastrointestinal stromal tumors, testicular seminoma, mast cell disease, melanoma, acute myeloid leukemia, while inactivating mutations are associated with the genetic defect piebaldism.

Immunogen: A synthetic peptide from the internal region of KIT which includes the deletion of V560, human origin.

Applications: ELISA, WB, IHC

Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes V560DEL mutant, but not wild-type KIT of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Download Datasheet:

Western blot:

Western blot analysis of recombinant KIT(V560DEL) and wild-type proteins. Purified His-tagged KIT(V560DEL) protein (lane 1) and corresponding wild-type protein (lane 2) were blotted with Anti KIT(V560DEL) monoclonal antibody (Cat. #26289).

Immunofluorescence:

Immunofluorescence of cells expressing KIT proteins with Anti KIT(V560DEL) antibody. HEK293T cells were transfected with pCDNA3-GFP KIT(WT) plasmid (left column) or pCDNA3-GFP KIT(V560DEL) plasmid (right column), then fixed and stained with Anti KIT(V560DEL) monoclonal antibody (Cat. #26289).

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