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Anti IDH1(R132P) Mouse Monoclonal Antibody

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IDH1(R132P)[26406][100ul]CatalogNumber:26406GeneSymbol:IDH2

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IDH1(R132P) [26406]
[100ul]

Catalog Number: 26406

Gene Symbol: IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH

Description: Anti IDH1(R132P) Mouse Monoclonal Antibody

Background: Isocitrate dehydrogenases (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations affecting Arg-132 are tissue-specific, and suggest that this residue plays a unique role in the development of high-grade gliomas. Mutations of Arg-132 to Cys, His, Leu or Ser abolish magnesium binding and abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.

Immunogen: A synthetic peptide from the internal region of IDH1 which includes the mutation of R132P, human origin.

Applications: ELISA, WB, IHC

Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: recognizes R132P mutant, but not wild-type IDH2 of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Download Datasheet:

Western blot:

Western blot analysis of recombinant IDH1(R132P) and wildtype proteins. Purified His-tagged IDH1(R132P) (lane 1) and corresponding wild-type IDH1 protein (lane 2) were blotted with Anti IDH1(R132P) monoclonal antibody (Cat. #26406).

Immunofluorescence:

Immunofluorescence of cells expressing IDH1 proteins with Anti IDH1(R132P) antibody. HEK293T cells were transfected with pCDNA3-GFP IDH1(R132P) plasmid, pCDNA3-GFP IDH1(WT) plasmid , pCDNA3-GFP IDH1(R132S) plasmid, pCDNA3-GFP IDH1(R132L) plasmid , pCDNA3-GFP IDH1(R132G) plasmid or pCDNA3-GFP IDH1(R132C) plasmid , then fixed and stained with Anti IDH1(R132P) monoclonal antibody (Cat. #26406).

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