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Orai1-L1

詳細(xì)介紹

Orai1-L1 [21001]
[100 µL]

Catalog Number: 21001

Gene Symbol: Orai1

Description: Anti Orai1 Rabbit Polyclonal Antibody (Orai1-L1)

Background: Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a ~35 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.

Immunogen: A peptide from the extracellular loop 1 region of human Orai1

Applications: ELISA, WB, IHC

Recommended Dilutions:
ELISA:      1:1000-1:5000,
WB:          1:1000-1:2000,
IHC:          1:100-1:200

Concentration:

Host: Rabbit

Format: Liquid

Clonality: Polyclonal

Isotype: IgG

Purity: Immunogen affinity purified

Storage buffer:

Preservative: 0.01% sodium azide

Constituents: 50mM NaHCO₃-Glycine, 0.15M NaCl, pH7.4

Species Reactivity: Orai1-L1 antibody recognizes Orai1 from human and mouse. It may also recognize Orai2 and Orai3.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Download Datasheet:

Western blot:

	Western blot analysis of Orai1 protein. Human Orai1 plasmid DNA was transiently transfected into HEK-293 cells. Whole-cell lysate was used for western blot in the absence (lane 1) or presence (lane 2) of antigen peptide.
Immunohistochemistry
	Immunohistochemistry of endogenous Orai1 in NIH3T3 cells showing the plasma membrane staining.

Publications:

1.
J Pharmacol Exp Ther. 2013 May;345(2):225-38

2.
Ann Allergy Asthma Immunol. 2012 Feb;108(2):103-12

3.
Am J Otolaryngol. 2011 Jul-Aug;32(4):318-28

4.
Histochem Cell Biol. 2010 Jul;134(1):93-102

5.
Cancer Cell. 2009 Feb 3;15(2):124-34

6.
Biotech Histochem. 2014 May;89(4):273-86

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