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Anti SUMO-1 Rabbit polyclonal Antibody

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Anti SUMO-1 Rabbit polyclonal Antibody

詳細(xì)介紹

SUMO-1 [21236]
[100 µL]

Catalog Number: 21236

Gene Symbol: SUMO1

Description: Anti SUMO-1 Rabbit polyclonal Antibody

Background: Small Ubiquitin-like Modifier or SUMO proteins are a family of small proteins that are covalently attached to and detached from other proteins in cells to modify their function. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle. There are 3 confirmed SUMO isoforms in humans: SUMO-1, SUMO-2 and SUMO-3. SUMO-2 and -3 show a high degree of similarity to each other and are distinct from SUMO-1.

Immunogen: A synthetic peptide corresponding to residues near the C-terminal of human SUMO-1.

Applications: ELISA, WB, IHC, IP

Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Rabbit

Format: Liquid

Clonality: Polyclonal

Isotype:

Purity: Purified from serum

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ ａnd Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Detects SUMO-1 but not SUMO-2/3 from vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Download Datasheet:

Western blot:

Western blot analysis of over-expressed SUMO-1 proteins. HEK293 cells were transfected with SUMO-1 (lane 2) and SUMO-2 (lane 3) cDNAs and control empty vector (lane 1). Cell lysates were blotted with anti SUMO-1 rabbit polyclonal antibody (Cat. # 21236).

Publications:

1.
Journal of Cellular Biochemistry Volume 114, Issue 10, pages 2323–2333, October 2013

2.
J Biol Chem. 2014 May 2;289(18):12666-78

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