L-929細(xì)胞-小鼠成纖維細(xì)胞
細(xì)胞名稱：L-929細(xì)胞-小鼠成纖維細(xì)胞
細(xì)胞別稱： NCTC 929; NCTC-929; L cell; L cells; L-cell; L-cells; L cell line; L; Strain L-929; L-929; L 929; L929; L929(NCTC);
Clone 929; Earles's cells; Earle's L cells
規(guī)格： 1X10^6| T25培養(yǎng)瓶
價(jià)格：1500元 (干冰運(yùn)輸需另加200元干冰費(fèi))
種屬來(lái)源：小鼠
組織來(lái)源：皮下結(jié)締組織
細(xì)胞形態(tài)：成纖維細(xì)胞樣
生長(zhǎng)特性：貼壁生長(zhǎng)
培養(yǎng)體系：MEM+10%FBS+1%P/S
L-929細(xì)胞-小鼠成纖維細(xì)胞 配套培養(yǎng)基貨號(hào)：TCM-G749
傳代比例：1:3-1:6，每2-3天換液一次
傳代周期：48-72 h
培養(yǎng)條件：氣相：95%空氣+5%CO2，溫度：37℃
凍存條件：60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO，液氮儲(chǔ)存
質(zhì)量檢測(cè)：細(xì)菌、真菌、支原體檢測(cè)均為陰性
1948年三月建立了NCTCclone929(小鼠結(jié)締組織)，細(xì)胞系L的克隆。細(xì)胞系L是較早建立的連續(xù)培養(yǎng)細(xì)胞系之一，而clone929是較早的克隆株。從一只100日齡的雄性C3H/An小鼠的正常皮下疏松結(jié)締組織入脂肪組織中建立了親本細(xì)胞系L。第95代的細(xì)胞系L使用毛細(xì)管法分離單細(xì)胞建立了clone929。
參考文獻(xiàn)：
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Sanford KK, et al. The growth in vitro of single isolated tissue cells. J. Natl. Cancer Inst. 9: 229-246, 1948.
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ASTM International Standard Practice for Direct Contact Cell Culture Evaluation of Materials for Medical Devices. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 0813-07.
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U.S. Pharmacopeia General Chapters:<87>Biological Reactivity Tests, in vitro. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011
Westfall BB, et al. The glycogen content of cell suspensions prepared from massive tissue culture: comparison of cells derived from mouse connective tissue and mouse liver. J. Natl. Cancer Inst. 14: 655-664, 1953. PubMed:
Earle WR, et al. Production of malignancy in vitro. IV. The mouse fibroblast cultures and changes seen in the living cells. J. Natl. Cancer Inst. 4: 165-212, 1943."
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