bv2小膠質(zhì)細(xì)胞

細(xì)胞名稱： 小鼠小膠質(zhì)細(xì)胞

細(xì)胞別稱： BV-2

細(xì)胞簡稱：bv2小膠質(zhì)細(xì)胞

貨號：TCM-C718

規(guī)格： 1X10^6

價(jià)格：1500元 (干冰運(yùn)輸需另加200元干冰費(fèi))

種屬來源：小鼠

組織來源：腦

細(xì)胞形態(tài)：多形型

生長特性：半貼壁生長

培養(yǎng)體系：DMEM-H+10%FBS+1%P/S

bv2小膠質(zhì)細(xì)胞 配套培養(yǎng)基貨號：TCM-G718

傳代比例：1:3-1:6，每2-3天換液一次

傳代周期：48-72 h

培養(yǎng)條件：氣相：95%空氣+5%CO2，溫度：37℃

凍存條件：60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO，液氮儲存

質(zhì)量檢測：細(xì)菌、真菌、支原體檢測均為陰性

bv2小膠質(zhì)細(xì)胞 要表達(dá)核v-myc、染色體v-raf癌基因，表面表達(dá)envgp70抗原，在形態(tài)學(xué)、表型及功能上有吞噬細(xì)胞的特征。

"Woo J.-M., Han D.-H., Park J.-H., Kim S.-J., Kim Y.-S.

In-depth characterization of the secretome of mouse CNS cell lines by LC-MS/MS without prefractionation.

Proteomics 15:3617-3622(2015)

Stansley B., Post J., Hensley K.

A comparative review of cell culture systems for the study of microglial biology in Alzheimer's disease.

J. Neuroinflamm. 9:115-115(2012)

Atwood B.K., Lopez J., Wager-Miller J., Mackie K., Straiker A.

Expression of G protein-coupled receptors and related proteins in HEK293, AtT20, BV2, and N18 cell lines as revealed by microarray analysis.

BMC Genomics 12:14-14(2011)

Crain J.M., Watters J.J.

Estrogen and P2 purinergic receptor systems in microglia: therapeutic targets for neuroprotection.

Open Drug Discov. J. 2:148-167(2010)

Henn A., Lund S., Hedtjarn M., Schrattenholz A., Porzgen P., Leist M.

The suitability of BV2 cells as alternative model system for primary microglia cultures or for animal experiments examining brain inflammation.

ALTEX 26:83-94(2009)"