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CRL-2784 2V6.11 [JHU-67] 人胚腎細(xì)胞ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞,。細(xì)胞庫管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和*培養(yǎng)條件，

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CRL-2784 2V6.11 [JHU-67] 人胚腎細(xì)胞 的詳細(xì)介紹

CRL-2784 2V6.11 [JHU-67] 人胚腎細(xì)胞
ATCC® Number: CRL-2784™
Designations: 2V6.11 [JHU-67]
Depositors: G Ketner
Biosafety Level: 2 [Cells containing Adenovirus viral DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens （human）
Morphology: epithelial

CRL-2784 2V6.11 [JHU-67] 人胚腎細(xì)胞
Source: Organ: kidney
Cell Type: epithelialtransformed with adenovirus 5 DNA
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: Part of the Johns Hopkins Special Collection
Isolation: Isolation date: 2001
Age: fetus
Comments: This line was derived from the human embryonic kidney line, 293 in 2001. 293 cells were transfected with the plasmid pVgRXR bearing a Zeocin-resistance selectable marker to obtain 293VgRXR cells. This population was subsequently transfected with the plasmid pEKORF6 containing Ad5 E4 ORF 6 that encodes E4 34K. pEKORF6 is a derivative of the plasmid pIndHydro that contains a hygromycin resistance gene. The vectors contain SV40 viral DNA sequences. The 2V6.11 cell line was originally selected in hygromycin-containing medium and cloned by single-colony isolation with a cloning cylinder. The 2V6.11 cells inducibly express the human adenovirus E4, 34kDa protein （E4 ORF6）. 2V6.11 cells exhibit little or no constitutive E4 biologic activity. Induction by ponasterone A, an ecdysone analog, however, results in E4 biologic activity and levels of E4 34kDa protein that are detectable by immunoblotting. These cells can be used as a tool to study the biology of the adenoviral E4 oncoprotein.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide （CO2）, 5%
Temperature: 37.0°C
Growth Conditions: The depositor recommends periodic hygromycin selection.
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% （w/v） Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）.
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
An inoculum of 5 X 10（3） to 1 X 10（4） viable cells/cm2 is recommended.
Incubate cultures at 37°C.

Interval: Maintain cultures at a cell concentration between 2 X 10（4） and 2 X 10（5） cells/cm2.
Subc*tion Ratio: A subc*tion ratio of 1:6 to 1:10 is recommended
Medium Renewal: Two to three times weekly
Preservation: Freeze medium: Complete growth medium supplemented with 5% （v/v） DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 23 hrs.
Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2003
recommended serum:ATCC 30-2020
source culture:ATCC JHU-67
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CRL-2784 2V6.11 [JHU-67] 人胚腎細(xì)胞

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iec-6 大鼠小腸隱窩上皮細(xì)胞
馬-達(dá)二氏犬腎細(xì)胞系 MDCK （NBL-2） CRL-34
鴨子胚胎成纖維細(xì)胞　 Duck embryo　 CCL-141
小鼠胚胎成纖維細(xì)胞 3T3-L1 CCL-173
人結(jié)腸直腸腺癌 COLO 320DM CCL-220
人結(jié)腸直腸腺癌 NCI-H508 CRL-253
人結(jié)腸直腸腺癌 COLO 320HSR CCL-220.1
人B淋巴細(xì)胞 Ramos（RA1） CRL-1596
人淋巴母細(xì)胞 T2（174×CEM.T2） CRL-1992
大鼠心肌細(xì)胞 H9C2（2-1） CRL-1446
豬鼻甲黏膜成纖維細(xì)胞 PT-K75 CRL-2528
小鼠胰腺癌細(xì)胞 LTPA CRL-2389
大鼠胰腺癌細(xì)胞 DSL-6A/C1 CRL-2132
小鼠骨髓瘤B淋巴細(xì)胞 SP2/MIL-6 CRL-2016
大鼠腦間質(zhì)細(xì)胞 DI TNC1 CRL-2005
小鼠肌原細(xì)胞 C2C12 CRL-1772
鵪鶉?yán)w維肉瘤細(xì)胞 QT6 CRL-1708
大鼠回腸細(xì)胞 IEC-18 CRL-1589
非洲綠猴腎細(xì)胞 VERO C1008 [Vero 76, clone E6, Vero E6] CRL-1586
人成纖維細(xì)胞 Hs 738.St/Int CRL-7869
人正常前列腺上皮細(xì)胞 WPE-int CRL-2888
人正常前列腺成肌纖維細(xì)胞 WPMY-1 CRL-2854
人胰腺導(dǎo)管腺癌細(xì)胞 PL45 CRL-2558
人胰腺癌細(xì)胞 Panc 05.04 CRL-2557
人胰腺癌細(xì)胞 Panc 04.03 CRL-2555
人胰腺癌細(xì)胞 Panc 02.03 CRL-2553
人胰腺癌細(xì)胞 Panc 08.13 CRL-2551

CRL-2784 2V6.11 [JHU-67] 人胚腎細(xì)胞
人胰腺癌細(xì)胞 Panc 03.27 CRL-2549
人胰腺癌細(xì)胞 Panc 10.05 CRL-2547
人肝癌細(xì)胞 SNU-398 CRL-2233
人成神經(jīng)瘤細(xì)胞-骨髓 SK-N-DZ CRL-2149
人胰腺癌細(xì)胞 HPAC CRL-2119
人胰腺癌細(xì)胞 HPAF-II CRL-1997
人黑色素瘤細(xì)胞 A375.S2 CRL-1872
人臍靜脈內(nèi)皮細(xì)胞 HUV-EC-C CRL-1730
人黑素瘤細(xì)胞 WM-266-4 CRL-1676
人膀胱癌細(xì)胞 HT-1376 CRL-1472
人正常前列腺上皮細(xì)胞 RWPE-2 CRL-11610
人正常前列腺上皮細(xì)胞 RWPE-1 CRL-11609
雞胚胎成纖維細(xì)胞 UMNSAH/DF-1 CRL-12203
人乳腺癌細(xì)胞 MDA-MB-231 HTB-26
小鼠巨噬細(xì)胞 RAW 264.7 TIB-71
人橫紋肌肉瘤細(xì)胞 A-204 HTB-82
人胰腺癌細(xì)胞 Capan-2 HTB-80
人胰腺癌細(xì)胞 Capan-1 HTB-79
人乳腺導(dǎo)管癌細(xì)胞 MDA-MB-435S HTB-129
人胃癌細(xì)胞 Hs 746T HTB-135
人肺腺癌細(xì)胞 NCI-H441 [H441] HTB-174
人骨髓瘤細(xì)胞 U266 TIB-196
人結(jié)腸癌細(xì)胞 NCI-H716 [H716] CCL-251
人多形性惡性膠質(zhì)瘤 T98G [T98-G] CRL-1690
人神經(jīng)母細(xì)胞瘤細(xì)胞 BE（2）-C CRL-2268
人腎透明細(xì)胞癌細(xì)胞 Caki-2 HTB-47
人皮膚成纖維細(xì)胞 Hs 865.Sk CRL-7601
CA46 人淋巴瘤細(xì)胞系
CRL-5826 NCI-H226 人肺鱗癌細(xì)胞系
Tca-8113 人舌鱗癌細(xì)胞
MA-891 小鼠乳腺癌高轉(zhuǎn)移細(xì)胞
U14 小鼠宮頸癌細(xì)胞
CRL-9518 L5178Y 小鼠淋巴瘤細(xì)胞
HTB-112 HEC-1-A 人子宮內(nèi)膜腺癌細(xì)胞
HTB-113 HEC-1-B 人子宮內(nèi)膜腺癌細(xì)胞
AN3 CA 人子宮內(nèi)膜腺癌細(xì)胞
ROS1728 大鼠成骨細(xì)胞
CRL-6323 B16F1 小鼠黑色素瘤細(xì)胞

CRL-2784 2V6.11 [JHU-67] 人胚腎細(xì)胞
HTB-58 SK-MES-1 人肺鱗癌細(xì)胞
CCL-127 IMR-32 人神經(jīng)母細(xì)胞瘤細(xì)胞
HTB-79 Capan-2 人胰腺癌細(xì)胞
BALL-1 人B淋巴細(xì)胞急性白血病細(xì)胞
CRL-2922 Eayh926 人臍靜脈細(xì)胞融合細(xì)胞
WB-F344 大鼠肝干細(xì)胞
CRL-1715 TM4 正常小鼠睪丸細(xì)胞
CRL-2539 4T1 小鼠乳腺癌細(xì)胞
CRL-1442 BRL-3A 大鼠肝細(xì)胞
BRL 大鼠肝細(xì)胞
RSC96大鼠雪旺細(xì)胞
Isk 人子宮內(nèi)膜癌細(xì)胞
HTB-113 hec-1-b 人子宮內(nèi)膜腺癌細(xì)胞
PLA-802?人惡性橫紋肌肉瘤
Pt-K2 袋鼠腎細(xì)胞
BS-C-1 非洲綠猴腎細(xì)胞
A498人腎癌細(xì)胞
3AO人卵巢癌細(xì)胞
iec-6 大鼠小腸隱窩上皮細(xì)胞
u87MG 人腦星形膠質(zhì)母細(xì)胞瘤

CRL-2784 2V6.11 [JHU-67] 人胚腎細(xì)胞
Renca 小鼠腎癌細(xì)胞
nci-h929 骨髓瘤細(xì)胞
P388小鼠白血病細(xì)胞
opm-2 骨髓瘤細(xì)胞
MDA-MB-435 人乳腺癌高轉(zhuǎn)移細(xì)胞
KU812 人外周血嗜堿性白細(xì)胞
NIH:OVCAR-3 人卵巢癌細(xì)胞
SW 1990人胰腺癌細(xì)胞系
S180人結(jié)腸腺癌細(xì)胞
HuTu-80十二指腸腺癌
RL95-2 人子宮內(nèi)腺癌細(xì)胞
NCI-H446 人小細(xì)胞肺癌細(xì)胞
MSTO-211H 人肺癌細(xì)胞
H4-Ⅱe 大鼠肝癌細(xì)胞系
HLF 人肺成纖維樣細(xì)胞
LS 180人結(jié)腸腺癌細(xì)胞
HuTu-80十一指腸腺癌
ST豬睪丸細(xì)胞系
REH人急性非B非T淋巴細(xì)胞白血病
NCI-H520 人肺鱗癌細(xì)胞
RKO 人結(jié)腸腺癌細(xì)胞
293A 人胚腎細(xì)胞
RK-13 兔腎細(xì)胞系