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HTB-174 NCI-H441 人肺腺癌細(xì)胞，原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種；細(xì)胞庫管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和*培養(yǎng)條件！

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HTB-174 NCI-H441 人肺腺癌細(xì)胞

ATCC^® Number:

HTB-174™

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$273.00

Designations:	NCI-H441 [H441]
Depositors:	AF Gazdar, JD Minna
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Homo sapiens （human）
Morphology:	epithelial
Source:	Organ: lung Disease: papillary adenocarcinoma
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation:	Isolation date: 1982
DNA Profile （STR）:	Amelogenin: X,Y CSF1PO: 11,12 D13S317: 9 D16S539: 9,13 D5S818: 11,12 D7S820: 10 THO1: 9.3 TPOX: 8,10 vWA: 17
Cytogenetic Analysis:	modal number = 52; range = 44 to 59. This is a hyperdiploid human cell line. The modal chromosome number was 52, but cells with 53 chromosome counts also occurred at a high frequency. The rate of cells with higher ploidies was 4.9%. Over 14 marker chromosomes were common to all cells, and 6 or more others were present in some cells. Among the common markers were: der（1）t（1;1）（p36;p21）, i（2q）, der（11）t（11;12）（q21;q13）, t（6p12q）, der（9）t（9;?;14）（p24;?q11）, t（12q22q） and 8 or more others. All these markers were present in a single copy per cell. Structurally normal N13 and N14 were absent; and N1, N6, N9, N12, N17, F and G chromosomes were single. A single copy each of the X and Y chromosome was detected. The presence of the Y chromosome was confirmed by fluorescent microscopy.
Isoenzymes:	AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 1 PGM3, 1
Gender:	male
Comments:	The NCI-H441 cell line was derived by A.F. Gazdar, M. Brower and D. Carney and associates in 1982 from the pericardial fluid of a patient with papillary adenocarcinoma of the lung. The cell line expresses mRNA and protein of the major surfactant apoprotein （SP-A）. Electron microscopy shows multilamellar bodies and cytoplasmic structures resembling clara cell granules. The cells can be cloned in soft agar with or without serum. The line has been used as a transfection host for expression of pulmonary surfactant protein （SP-B）.
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Growth Conditions: :If desired, the serum can be reduced to 5%.
Subculturing:	Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% （w/v） Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）. Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. *Subction Ratio:** A subction ratio of 1:3 to 1:8 is recommended Medium Renewal:* 2 to 3 times per week
Preservation:	Freeze medium: Complete growth medium supplemented with 5% （v/v） DMSO Storage temperature: liquid nitrogen vapor phase
Doubling Time:	58 hrs in medium with serum; 99 to 138 hrs in serum-free medium
Related Products:	Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2001 recommended serum:ATCC 30-2020
References:	22250: Bepler G, et al. Expression of p64c-myc and neuroendocrine properties define three subclasses of small cell lung cancer. Oncogene 4: 45-50, 1989. PubMed: 2536917 22434: Brower M, et al. Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium. Cancer Res. 46: 798-806, 1986. PubMed: 3940644 22465: Broers JL, et al. Spontaneous changes in intermediate filament protein expression patterns in lung cancer cell lines. J. Cell Sci. 91: 91-108, 1988. PubMed: 2473086 22705: O'Reilly MA, et al. Differential effects of glucocorticoid on expression of surfactant proteins in a human lung adenocarcinoma cell line. Biochim. Biophys. Acta 970: 194-204, 1988. PubMed: 3382698 22998: O'Reilly MA, et al. In vitro translation, post-translational processing and secretion of pulmonary surfactant protein B precursors. Biochim. Biophys. Acta 1011: 140-148, 1989. PubMed: 2713400 23081: Gazdar AF, et al. Peripheral airway cell differentiation in human lung cancer cell lines. Cancer Res. 50: 5481-5487, 1990. PubMed: 2386953 23342: Baatz JE, et al. Utilization of modified surfactant-associated protein B for delivery of DNA to airway cells in culture. Proc. Natl. Acad. Sci. USA 91: 2547-2551, 1994. PubMed: 8146151 24389: . . Lung Cancer 4: 155-161, 1988. 32583: Tamura T, Stadtman TC. A new selenoprotein from human lung adenocarcinoma cells: purification, properties, and thioredoxin reductase activity. Proc. Natl. Acad. Sci. USA 93: 1006-1011, 1996. PubMed: 8577704 32830: Yamaguchi Y, et al. Biochemical characterization and intracellular localization of the Menkes disease protein. Proc. Natl. Acad. Sci. USA 93: 14030-14035, 1996. PubMed: 8943055

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人淋巴母細(xì)胞 T2（174×CEM.T2） CRL-1992
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HTB-174 NCI-H441 人肺腺癌細(xì)胞
人正常前列腺成肌纖維細(xì)胞 WPMY-1 CRL-2854
人胰腺導(dǎo)管腺癌細(xì)胞 PL45 CRL-2558
人胰腺癌細(xì)胞 Panc 05.04 CRL-2557
人胰腺癌細(xì)胞 Panc 04.03 CRL-2555
人胰腺癌細(xì)胞 Panc 02.03 CRL-2553
人胰腺癌細(xì)胞 Panc 08.13 CRL-2551
人胰腺癌細(xì)胞 Panc 03.27 CRL-2549
人胰腺癌細(xì)胞 Panc 10.05 CRL-2547
人肝癌細(xì)胞 SNU-398 CRL-2233
人成神經(jīng)瘤細(xì)胞-骨髓 SK-N-DZ CRL-2149
人胰腺癌細(xì)胞 HPAC CRL-2119
人胰腺癌細(xì)胞 HPAF-II CRL-1997
人黑色素瘤細(xì)胞 A375.S2 CRL-1872
人臍靜脈內(nèi)皮細(xì)胞 HUV-EC-C CRL-1730
人黑素瘤細(xì)胞 WM-266-4 CRL-1676
人膀胱癌細(xì)胞 HT-1376 CRL-1472
人正常前列腺上皮細(xì)胞 RWPE-2 CRL-11610
人正常前列腺上皮細(xì)胞 RWPE-1 CRL-11609
雞胚胎成纖維細(xì)胞 UMNSAH/DF-1 CRL-12203
人乳腺癌細(xì)胞 MDA-MB-231 HTB-26
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人胰腺癌細(xì)胞 Capan-2 HTB-80

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人結(jié)腸癌細(xì)胞 NCI-H716 [H716] CCL-251
人多形性惡性膠質(zhì)瘤 T98G [T98-G] CRL-1690
人神經(jīng)母細(xì)胞瘤細(xì)胞 BE（2）-C CRL-2268
人腎透明細(xì)胞癌細(xì)胞 Caki-2 HTB-47
人皮膚成纖維細(xì)胞 Hs 865.Sk CRL-7601
CA46 人淋巴瘤細(xì)胞系
CRL-5826 NCI-H226 人肺鱗癌細(xì)胞系
Tca-8113 人舌鱗癌細(xì)胞
MA-891 小鼠乳腺癌高轉(zhuǎn)移細(xì)胞
U14 小鼠宮頸癌細(xì)胞
CRL-9518 L5178Y 小鼠淋巴瘤細(xì)胞
HTB-112 HEC-1-A 人子宮內(nèi)膜腺癌細(xì)胞
HTB-113 HEC-1-B 人子宮內(nèi)膜腺癌細(xì)胞
AN3 CA 人子宮內(nèi)膜腺癌細(xì)胞
ROS1728 大鼠成骨細(xì)胞

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CCL-127 IMR-32 人神經(jīng)母細(xì)胞瘤細(xì)胞
HTB-79 Capan-2 人胰腺癌細(xì)胞
BALL-1 人B淋巴細(xì)胞急性白血病細(xì)胞
CRL-2922 Eayh926 人臍靜脈細(xì)胞融合細(xì)胞
WB-F344 大鼠肝干細(xì)胞
CRL-1715 TM4 正常小鼠睪丸細(xì)胞
CRL-2539 4T1 小鼠乳腺癌細(xì)胞
CRL-1442 BRL-3A 大鼠肝細(xì)胞
BRL 大鼠肝細(xì)胞
RSC96大鼠雪旺細(xì)胞
Isk 人子宮內(nèi)膜癌細(xì)胞
HTB-113 hec-1-b 人子宮內(nèi)膜腺癌細(xì)胞
PLA-802?人惡性橫紋肌肉瘤
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BS-C-1 非洲綠猴腎細(xì)胞
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opm-2 骨髓瘤細(xì)胞
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S180人結(jié)腸腺癌細(xì)胞
HuTu-80十二指腸腺癌
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NCI-H446 人小細(xì)胞肺癌細(xì)胞
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LS 180人結(jié)腸腺癌細(xì)胞
HuTu-80十一指腸腺癌
ST豬睪丸細(xì)胞系
REH人急性非B非T淋巴細(xì)胞白血病
NCI-H520 人肺鱗癌細(xì)胞
RKO 人結(jié)腸腺癌細(xì)胞
293A 人胚腎細(xì)胞
RK-13 兔腎細(xì)胞系