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更新時間:2024-04-29 09:45:38瀏覽次數(shù):33次
聯(lián)系我時,請告知來自 環(huán)保在線PIK3CA(E545G)[26208][100ul]CatalogNumber:26208GeneSymbol:PIK3CA
PIK3CA(E545G) [26208]
[100ul]
Catalog Number: 26208 |
Gene Symbol: PIK3CA, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha |
Description: Anti PIK3CA(E545G) Mouse Monoclonal Antibody |
Background: Phosphatidyl 3-kinases (PI3K) are a family of lipid kinases involved in many cellular processes, including cell growth, proliferation, differentiation, motility, and survival. It is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Mutants of PIK3CA has been found in the pathogenesis of several cancers, including colon cancer, gliomas, gastric cancer, breast cancer, endometrial cancer, and lung cancer. |
Immunogen: A synthetic peptide from the internal region of PIK3CA which includes the mutation of E545G, human origin. |
Applications: ELISA, WB, IHC |
Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
Concentration: 1 mg/ml |
Host: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: |
Purity: Purified from ascites |
Storage buffer: |
Preservative: no |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Recognizes E545G mutant, but not wild-type PIK3CA of vertebrates. |
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
Download Datasheet: |
Western blot: |
Western blot analysis of recombinant PIK3CA(E545G) and wildtype proteins. Purified His-tagged PIK3CA(E545G) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with Anti PIK3CA(E545G) monoclonal antibody (Cat. #26208). |
Immunofluorescence: |
Immunofluorescence of cells expressing PIK3CA proteins with Anti PIK3CA(E545G) antibody. HEK293T cells were transfected with pCDNA3-GFP PIK3CA(WT) plasmid (left column) or pCDNA3-GFP PIK3CA(E545G) plasmid (right column), then fixed and stained with Anti PIK3CA(E545G) monoclonal antibody (Cat. #26208). |
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Anti PIK3CA(E545K) Rabbit Polyclonal Antibody | 100 μL | ¥4,800 | |
Anti PIK3CA Mouse Monoclonal Antibody | 100 μL | ¥2,800 | |
Anti PIK3CA(E545G) Mouse Monoclonal Antibody | 100 μL | ¥4,800 | |
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Anti PIK3CA(E542Q) Mouse Monoclonal Antibody | 100 μL | ¥4,800 | |
Anti PIK3CA(Q546K) Mouse Monoclonal Antibody | 100 μL | ¥4,800 | |
Anti PIK3CA(H1047R) Rabbit Polyclonal Antibody | 100 μL | ¥4,800 | |
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