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Anti PIK3CA(E545G) Mouse Monoclonal Antibody

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參   考   價: 4800

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更新時間:2024-04-29 09:45:38瀏覽次數(shù):33次

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所在地區(qū):湖北武漢市

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PIK3CA(E545G)[26208][100ul]CatalogNumber:26208GeneSymbol:PIK3CA

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PIK3CA(E545G) [26208]
[100ul]

Catalog Number:  26208
Gene Symbol:  PIK3CA, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha
Description:   Anti PIK3CA(E545G) Mouse Monoclonal Antibody
Background:   Phosphatidyl 3-kinases (PI3K) are a family of lipid kinases involved in many cellular processes, including cell growth, proliferation, differentiation, motility, and survival. It is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Mutants of PIK3CA has been found in the pathogenesis of several cancers, including colon cancer, gliomas, gastric cancer, breast cancer, endometrial cancer, and lung cancer.
Immunogen:   A synthetic peptide from the internal region of PIK3CA which includes the mutation of E545G, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:500-1:1000 
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes E545G mutant, but not wild-type PIK3CA of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
Download Datasheet:  
Western blot:  
Western blot analysis of recombinant PIK3CA(E545G) and wildtype proteins. Purified His-tagged PIK3CA(E545G) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with Anti PIK3CA(E545G) monoclonal antibody (Cat. #26208).
Immunofluorescence:  
Immunofluorescence of cells expressing PIK3CA proteins with Anti PIK3CA(E545G) antibody. HEK293T cells were transfected with pCDNA3-GFP PIK3CA(WT) plasmid (left column) or pCDNA3-GFP PIK3CA(E545G) plasmid (right column), then fixed and stained with Anti PIK3CA(E545G) monoclonal antibody (Cat. #26208).


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